FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672857
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34650.5149682622013296No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA27220.404543610306499No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCCCAG21300.31656057676445365No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA20480.30437373765896764No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA19620.29159241859711643No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAA17810.2646922005715925No Hit
CATTTAAATCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT17450.25934188096430594No Hit
GTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAAACAATATGTT16550.24596608194608957No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT16510.24537160198972444No Hit
CCCCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15350.22813168325513444No Hit
CCTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTG15010.2230786036260305No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA14660.21787690400783527No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG14430.2144586442587355No Hit
ATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT14320.2128238243787313No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC13270.19721872552414554No Hit
AGTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGATTTAAATGTATA13250.19692148554596298No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG13220.1964756255786891No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13180.19588114562232392No Hit
GGGTAAAGGTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGA12850.19097668598231124No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12790.19008496604776348No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAT12670.188301526178668No Hit
GTGCTGGTGGACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12540.18636946632048118No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG12030.17878984687682523No Hit
CCTGTAAACAGGCCTATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCT11870.17641192705136455No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTTGACACACC11700.17388538723681257No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC11400.16942678756407378No Hit
CCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA11170.16600852781497405No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCA11090.16481956790224372No Hit
GTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA10550.1567940884913139No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT10550.1567940884913139No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT10500.15605098854585744No Hit
GGATAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAAT10380.15426754867676193No Hit
CTCTAAGGCCTCCCGATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAA10140.1507006689385709No Hit
TCCCGATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA10100.15010618898220573No Hit
GACAAAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA10090.14995756899311444No Hit
CCTTACCCCAGGAACATATTGTTTTGAAAATCAAACAATGTTTTAGAAAA10050.14936308903674925No Hit
TAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG9930.14757964916765376No Hit
ATGTAATTGGAAGTTGGGGCACCTTACCCCAGGAACATATTGTTTTGAAA9930.14757964916765376No Hit
ATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAAGAAGT9450.1404458896912717No Hit
GTTAATAGGTGTAAACAATTTGTGGGGCCCCTCACAGTAAATGAAAACAG9320.13851382983308488No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC9270.13777072988762842No Hit
GTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA9270.13777072988762842No Hit
GTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGATTTAAATGTATAC9190.13658176997489807No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAACGCCG9190.13658176997489807No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG9080.13494695009489385No Hit
GATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9050.13450109012762No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8940.13286627024761577No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATATGAAACCACA8910.13242041028034188No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG8780.13048835042215506No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG8620.12811043059669439No Hit
GGCCTATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCTTTTGGGATTT8560.12721871066214663No Hit
GTCTTTGGGTATACATTTAAATCCTCATAAAACAAAAAGATGGGGCTACT8510.12647561071669017No Hit
ACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTT8460.1257325107712337No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8410.12498941082577725No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACA8290.12320597095668173No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCCT8260.12276011098940785No Hit
GGGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8100.12038219116394717No Hit
ATATGTTCCTGGGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAA7890.11726117139303001No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT7880.11711255140393872No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7870.11696393141484743No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7850.11666669143666483No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT7810.11607221148029968No Hit
GACGGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA7730.11488325156756933No Hit
GGTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7690.11428877161120415No Hit
CATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAA7650.11369429165483899No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7640.11354567166574771No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7620.11324843168756511No Hit
CCTATTAACAATGTCCTCCTGCAAATGAATATCAGGAAAAGAAGGGGTTT7560.11235671175301735No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAA7460.11087051186210443No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG7330.10893845200391764No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA7260.10789811208027857No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7240.10760087210209598No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG7170.10656053217845693No Hit
TTTAAATCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCATGG7150.10626329220027435No Hit
CATTAGGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCA7090.1053715722657266No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7090.1053715722657266No Hit
GCTATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT6980.10373675238572239No Hit
TCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAAAGGACGACAGAAT6920.10284503245117463No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAG6820.10135883256026168No Hit
GCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGG6810.10121021257117041No Hit
GGAACATATTGTTTTGAAAATCAAACAATGTTTTAGAAAACTTCCTGTAA6800.1010615925820791No Hit
GGATATGTAATTGGAAGTTGGGGCACCTTACCCCAGGAACATATTGTTTT6780.10076435260389652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)