FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672857
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34440.5118472424304124No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22620.3361784153245043No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCCCAG21660.3219108963717402No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA20100.2987261780734985No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA19850.2950106783462162No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT18630.2768790396770785No Hit
CATTTAAATCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT17530.26053084087703626No Hit
GTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAAACAATATGTT17400.2585987810188495No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAA15790.23467096277515134No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC15400.22887478320059093No Hit
CCTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTG14330.21297244436782262No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA13720.2039066250332537No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAT13320.197961825469602No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13250.19692148554596298No Hit
CCCCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13150.19543528565505003No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12800.19023358603685478No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG12600.18726118625502894No Hit
ATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT12450.18503188641865953No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG12180.18101914671319463No Hit
GGGTAAAGGTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGA11830.17581744709499939No Hit
AGTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGATTTAAATGTATA11640.17299366730226484No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC11620.17269642732408225No Hit
GTGCTGGTGGACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11550.17165608740044316No Hit
GTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTA11350.16868368761861732No Hit
GTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCGTTGACACACC10950.1627388880549656No Hit
GGGCTGTACCGGCCGCTTCTCCGTCTGCCGTACCGACCGACCACGGGGCG10540.1566454685022226No Hit
CCTTACCCCAGGAACATATTGTTTTGAAAATCAAACAATGTTTTAGAAAA10260.1524841088076664No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT10260.1524841088076664No Hit
ATGTAATTGGAAGTTGGGGCACCTTACCCCAGGAACATATTGTTTTGAAA10160.1509979089167535No Hit
CCTGTAAACAGGCCTATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCT10070.14966032901493184No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCA9880.1468365492221973No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG9850.1463906892549234No Hit
ATATGTTCCTGGGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAA9840.1462420692658321No Hit
GTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA9630.14312104949491497No Hit
GGATAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAAT9440.1402972697021804No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT9310.13836520984399361No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG8990.13360937019307223No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA8970.13331213021488963No Hit
GTCTTTGGGTATACATTTAAATCCTCATAAAACAAAAAGATGGGGCTACT8850.13152869034579412No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8770.1303397304330638No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC8760.1301911104439725No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8710.12944801049851604No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACA8670.12885353054215085No Hit
TAACCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG8630.12825905058578568No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATATGAAACCACA8570.12736733065123795No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8420.12513803081486854No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCATATTTCCT8340.12394907090213819No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC8290.12320597095668173No Hit
GGCCTATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCTTTTGGGATTT8210.12201701104395139No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8200.12186839105486011No Hit
GTTAATAGGTGTAAACAATTTGTGGGGCCCCTCACAGTAAATGAAAACAG8060.119787711207582No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAACGCCG8060.119787711207582No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8050.11963909121849071No Hit
GACAAAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA8020.11919323125121682No Hit
ATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAAGAAGT8020.11919323125121682No Hit
CTCTAAGGCCTCCCGATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAA7980.11859875129485165No Hit
GTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGATTTAAATGTATAC7860.11681531142575614No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT7780.11562635151302579No Hit
TCCCGATACAGAGCAGTGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA7610.11309981169847381No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7540.11205947177483477No Hit
CTTTTATATAAGATACCTGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7390.10983017193846537No Hit
GGATATGTAATTGGAAGTTGGGGCACCTTACCCCAGGAACATATTGTTTT7390.10983017193846537No Hit
GATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7250.10774949209118728No Hit
GACGGAAACTGCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAA7210.1071550121348221No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC7120.10581743223300047No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG7120.10581743223300047No Hit
GGAACATATTGTTTTGAAAATCAAACAATGTTTTAGAAAACTTCCTGTAA7120.10581743223300047No Hit
GGTTCAGATACTGTTTGTTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7060.10492571229845271No Hit
CTATTGTGGTTTCATATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7010.10418261235299625No Hit
TTTAAATCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCATGG6900.10254779247299203No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAA6890.10239917248390074No Hit
CCTATTAACAATGTCCTCCTGCAAATGAATATCAGGAAAAGAAGGGGTTT6870.10210193250571814No Hit
CATTAGGATAGAACCTAGCAGGCATGATCAATTTTAGTCTCCTGTTTTCA6730.10002125265844006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)