FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525951
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99431.890480291890309No Hit
ATCCTAACCTTACCAAATATTTGCCCCTGGACAAAGGCATTAAACCGTAT17580.3342516698323608No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16950.32227336767113285No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14990.2850075387250904No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11260.21408838465940744No Hit
GGTTAGGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11240.21370812109873352No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCCTGGACAAAGGCATTAAAC9980.18975151677627763No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9030.17168899764426726No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8910.16940741628022382No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA8780.16693570313584344No Hit
GGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8140.15476726919427855No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7850.14925344756450695No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7720.1467817344201266No Hit
GGATAATACGGTTTAATGCCTTTGTCCAGGGGCAAATATTTGGTAAGGTT7280.13841593608530073No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7240.13765540896395292No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTAGTACAATATGGT7070.13442316869822474No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7010.13328237801620305No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6730.1279586881667684No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6710.1275784246060945No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6690.1271981610454206No Hit
ATGTTAATATGGGCCTCAAAATAAGACAACTATTGTGGTTTCACATTTCC6550.12453631612070325No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6530.12415605256002936No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAGGGGCAAATATTTGGTA5880.1117974868381275No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5780.10989616903475799No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC5710.10856524657239933No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC5680.10799485123138847No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5660.10761458767071458No Hit
GTACAATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5530.1051428745263342No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5480.10419221562464945No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5430.10324155672296467No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5420.10305142494262773No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGG5380.10229089782127992No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTG5350.10172050248026906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)