FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525951
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36490.6937908664495362No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18430.3504128711610017No Hit
ATCCTAACCTTACCAAATATTTGCCCCTGGACAAAGGCATTAAACCGTAT17460.3319700884683174No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17050.32417468547450234No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12640.24032657034590674No Hit
GGTTAGGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11690.22226405121389634No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCCTGGACAAAGGCATTAAAC10360.1969765244290818No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9260.17606202859201714No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8790.1671258349161804No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA8220.15628832343697416No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7990.15191529248922428No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTAGTACAATATGGT7950.15115476536787648No Hit
GGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7930.15077450180720256No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7570.14392975771507233No Hit
ATGTTAATATGGGCCTCAAAATAAGACAACTATTGTGGTTTCACATTTCC7450.1416481763510289No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7330.13936659498698548No Hit
GGATAATACGGTTTAATGCCTTTGTCCAGGGGCAAATATTTGGTAAGGTT7210.13708501362294206No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7160.13613435472125732No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7070.13442316869822474No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6940.13195145555384438No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAGGGGCAAATATTTGGTA6680.12700802926508364No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6550.12453631612070325No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6160.11712117668756215No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC6140.11674091312688824No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTAGTACAATA6080.11560012244486655No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6030.11464946354318177No Hit
GTACAATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6000.11407906820217094No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC5880.1117974868381275No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGG5870.11160735505779056No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5860.11141722327745361No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5710.10856524657239933No Hit
ATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5610.1066639287690298No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGA5500.10457247918532334No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5380.10229089782127992No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5370.10210076604094298No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5310.10095997535892126No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5280.1003895800179104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)