FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419111
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17490.4173118815779113No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10780.25721109682160576No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG10590.2526776915900561No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACTATTCTTTGACATACT10430.24886008718454064No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10210.24361088112695684No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9760.23287386873644453No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9180.2190350527664509No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8880.21187704450610936No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8760.20901384120197275No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8530.20352603486904425No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8490.20257163376766538No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8470.20209443321697593No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8150.19445922440594496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8110.1935048233045661No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT8020.19135742082646365No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7870.18777841669629286No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7740.18467661311681152No Hit
GTCCCGTACTGGTAGTTGAGGTTCCTGGAAGTAGAGGACAAACGGGCAAC7710.18396081229077738No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7450.17775720513181473No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA7320.1746554015523334No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7270.1734624001756098No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTCAATTTCAT7210.17203079852354147No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6870.16391838916182108No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6550.1562831803507901No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6480.1546129784233771No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6470.15437437814803237No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCT6440.15365857732199822No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6420.1531813767713088No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6280.14984097291648274No Hit
CAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6250.1491251720904486No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAATA6030.14387596603286482No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG6010.14339876548217537No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6010.14339876548217537No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5700.13600215694648912No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5620.13409335474373138No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5570.13290035336700778No Hit
ATATTGTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA5530.1319459522656289No Hit
GCCCAAAAGACCCACTATTCTTTGACATACTTTCCAATCAATAGGTCTAT5480.13075295088890532No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5430.12955994951218175No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5410.1290827489614923No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5380.12836694813545815No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5380.12836694813545815No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5350.12765114730942398No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC5220.12454934372994265No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA5220.12454934372994265No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5200.12407214317925322No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTCAATTTCATGGGATATGTAA5110.12192474070115078No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5010.1195387379477036No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4860.11595973381753283No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAAT4860.11595973381753283No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACATATTGTACTCAAA4820.11500533271615394No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4810.11476673244080925No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4790.11428953189011981No Hit
AACATATTGTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAAT4710.11238072968736207No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4670.11142632858598318No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAAG4650.11094912803529375No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAATATGTT4620.11023332720925959No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4620.11023332720925959No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4610.10999472693391489No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGT4600.10975612665857015No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4580.10927892610788073No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC4550.10856312528184657No Hit
CTATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTT4540.10832452500650186No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4510.10760872418046771No Hit
GGTCTCTACCGTCCCCTGCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4500.10737012390512297No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4450.10617712252839939No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4450.10617712252839939No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4440.10593852225305468No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4320.10307531894891808No Hit
GGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTA4280.1021209178475392No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4210.10045071592012618No Hit
GTATACATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTCAAT4200.10021211564478144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)