FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705460
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG62670.8883565333257731No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA44230.6269668018030788No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT38940.5519802681938026No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG36960.5239134748958126No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT26540.3762084313781079No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT21190.30037138888101383No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT20880.29597709296062147No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA20370.2887477674141695No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA19600.27783290335384003No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA19420.2752813766903864No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18640.26422476114875404No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG18600.26365775522354207No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA18170.25756244152751395No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC17880.2534516485697276No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA16560.23474045303773422No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG16050.22751112749128227No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC16010.22694412156607038No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15840.2245343463839197No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14930.21163496158534859No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14440.2046891390015026No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14070.19944433419329233No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13940.19760156493635359No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC13910.19717631049244463No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13280.18824596717035694No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA12750.180733138661299No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC12620.1788903694043603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12600.1786068664417543No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG12020.1703852805261815No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11990.16996002608227256No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG11810.1674084994188189No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11790.16712499645621298No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11780.16698324497491No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC11770.166841493493607No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA11510.16315595497972954No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG11490.16287245201712358No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG10880.15422561165764184No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG10810.15323335128852097No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10650.15096532758767328No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC10630.15068182462506732No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA10540.14940606129334053No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAAT10430.14784679499900774No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10220.14487001389164517No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT10130.14359425055991837No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC10090.14302724463470642No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10060.1426019901907975No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC10040.14231848722819154No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA9960.1411844753777677No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9930.14075922093385876No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT9880.14005046352734385No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT9870.13990871204604088No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9860.13976696056473792No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA9840.13948345760213193No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT9760.1383494457517081No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGATAGTCCAA9660.1369319309386783No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9660.1369319309386783No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9280.1315453746491651No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9230.1308366172426502No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC9210.13055311428004424No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG9160.12984435687352933No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG9030.12800158761659058No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8550.12119751651404756No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8520.12077226207013864No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT8410.11921299577580585No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8260.11708672355626117No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8210.11637796614974627No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG8150.11552745726192838No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT8050.11410994244889859No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8030.11382643948629263No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC8030.11382643948629263No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7860.11141666430414197No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7780.11028265245371814No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT7680.10886513764068835No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7670.10872338615938537No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA7670.10872338615938537No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7660.10858163467808238No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7500.10631361097723471No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT7350.10418733875769003No Hit
CTCTAAGGCCTCCCGATACAGAGCGGAGGCGGTGTCGAGGAGATCTCGAA7340.10404558727638703No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7270.10305332690726618No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG7230.10248632098205426No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA7160.1014940606129334No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC7140.10121055765032745No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT7080.10036004876250956No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG7080.10036004876250956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)