FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547919
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC26480.4832831130148799No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21790.39768651935778826No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA19820.3617322998472402No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16480.3007743845349404No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACTTTTGG16200.2956641401375021No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC15820.28872880845526433No Hit
TTAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGT14490.2644551475674324No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA14380.26244755155415306No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC13760.25113201038839683No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13250.2418240652359199No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12480.22777089314296456No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG12400.22631082331512503No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT12070.22028803527528704No Hit
CTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT12000.21901047417592748No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11930.21773291307656792No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG11790.21517779087784875No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11630.21225765122216972No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG11580.21134510757977No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT11540.21061507266585022No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG11390.20787744173865114No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10960.20002956641401373No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG10800.1971094267583347No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10530.19218169108937636No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA10360.1890790427052174No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10250.18707144669193804No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9940.18141367610905992No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9870.18013611500970034No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9770.17831102772490093No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA9550.17429583569834228No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACTTTTGG9480.1730182745989827No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9400.17155820477114317No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT9340.17046315240026355No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9170.16736050401610456No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8570.1564099803073082No Hit
TTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGC8430.15385485810858904No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8300.15148224463834983No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATCTTT8200.14965715735355045No Hit
CGTAAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCTAGC8160.1489271224396307No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA8080.14746705261179116No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC8000.14600698278395163No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7940.144911930413072No Hit
ATTTATGCCTACAGCCTCCTAGTACATAAATCTTTAACCTAATCTCCTCC7920.1445469129561121No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7900.14418189549915222No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7780.141991790757393No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGTCCCACAATTCTTTGACATACT7610.138889142373234No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7530.13742907254539447No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTA7460.1361515114460349No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7420.13542147653211517No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7420.13542147653211517No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7150.13049374086315677No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG7030.12830363612139753No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6960.12702607502203794No Hit
GTATACATGCATATAAAGGCATTAATGCAGGATAGCCACATTGTGTAAAA6950.126843566293558No Hit
TTCCTAAAACATTGCTTGATTTTTTGTACAATATGTTCCTGCGGTAAAGT6900.1259310226511583No Hit
GGATTAAAGACAGGTACGGAAGAAGAATAAAGTCCAGTAAAGTTTCCCAC6880.12556600519419842No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6810.12428844409483883No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6770.12355840918091908No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGATTCCCGAGATTGAGATCT6730.12282837426699932No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6670.12173332189611967No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6590.12027325206828017No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC6550.11954321715436042No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6530.11917819969740054No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6480.11826565605500083No Hit
CTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG6430.11735311241260113No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6420.11717060368412119No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6410.11698809495564125No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC6400.11680558622716132No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6290.11479799021388197No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6280.11461548148540203No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6230.11370293784300234No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6070.11078279818732331No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6060.11060028945884337No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5990.1093227283594838No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5870.1071326236177245No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT5870.1071326236177245No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAAG5830.10640258870380476No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACC5800.10585506251836493No Hit
GCGGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC5760.10512502760444517No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5740.10476001014748532No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5730.10457750141900538No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5720.10439499269052543No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5700.10402997523356555No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5680.10366495777660567No Hit
GTCCAAGGGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5680.10366495777660567No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5630.10275241413420597No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5580.10183987049180628No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5560.1014748530348464No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT5550.10129234430636645No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5550.10129234430636645No Hit
TTCCTAAAACATTGCTTGAGTTTTTGTACAATATGTTCCTGCGGTAAAGT5520.10074481812092663No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5500.10037980066396675No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5500.10037980066396675No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5490.1001972919354868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)