FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547919
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC23210.4236027588019397No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21790.39768651935778826No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACTTTTGG17940.3274206588930116No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15330.27978588075974736No Hit
TTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGC14990.27358058399142937No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14360.2620825340971932No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC14000.2555122198719154No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA13420.24492671362007887No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12970.23671382083848158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12540.22886594551384418No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT12350.22539827967272535No Hit
TTAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGT12150.22174810510312654No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12130.22138308764616665No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG11370.20751242428169125No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11290.20605235445385175No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG11180.20404475844057243No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG11050.20167214497033323No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10930.19948204022857394No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCGAAAGATA10800.1971094267583347No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10590.19327674346025597No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10220.18652392050649821No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10180.18579388559257845No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT10020.18287374593689942No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT9970.18196120229449975No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACTTTTGG9810.1790410626388207No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9760.17812851899642101No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9750.17794601026794107No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9660.1763034317116216No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9410.1717407134996231No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9240.16863806511546414No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9140.16681297783066476No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9090.16590043418826506No Hit
CTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT9080.16571792545978511No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9020.16462287308890547No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC8290.1512997359098699No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8290.1512997359098699No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGTCCCACAATTCTTTGACATACT7990.1458244740554717No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7650.13961917728715376No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7590.1385241249162741No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7580.13834161618779417No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7460.1361515114460349No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7360.13432642416123552No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7350.13414391543275558No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7280.132866354333396No Hit
CTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACT7270.13268384560491606No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7220.13177130196251635No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTA7050.12866865357835738No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7010.12793861866443765No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6990.12757360120747777No Hit
GTATACATGCATATAAAGGCATTAATGCAGGATAGCCACATTGTGTAAAA6970.12720858375051788No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6910.12611353137963824No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC6880.12556600519419842No Hit
GGATTAAAGACAGGTACGGAAGAAGAATAAAGTCCAGTAAAGTTTCCCAC6870.12538349646571847No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGATTCCCGAGATTGAGATCT6740.12301088299547926No Hit
CGTAAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCTAGC6700.12228084808155949No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTG6600.12045576079676011No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6590.12027325206828017No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6560.11972572588284036No Hit
CCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG6480.11826565605500083No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATCTTT6440.11753562114108107No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6410.11698809495564125No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6380.11644056877020144No Hit
TTCCTAAAACATTGCTTGATTTTTTGTACAATATGTTCCTGCGGTAAAGT6350.11589304258476162No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6290.11479799021388197No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6280.11461548148540203No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA6230.11370293784300234No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6220.1135204291145224No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6200.11315541165756252No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT6140.1120603592866829No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACC6140.1120603592866829No Hit
GCGGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC6130.11187785055820296No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG6060.11060028945884337No Hit
CTTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG6040.11023527200188349No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAAG6020.1098702545449236No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTTCCG6000.10950523708796374No Hit
ATTTATGCCTACAGCCTCCTAGTACATAAATCTTTAACCTAATCTCCTCC5990.1093227283594838No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5980.10914021963100386No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTCTGCCAAGTGTTTGCTG5940.1084101847170841No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5930.10822767598860415No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC5860.10695011488924458No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5850.10676760616076464No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG5830.10640258870380476No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5750.10494251887596526No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC5680.10366495777660567No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5660.10329994031964579No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5630.10275241413420597No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5600.10220488794876616No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5570.10165736176332633No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG5490.1001972919354868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)