FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622143
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23580.3790125421325965No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20180.3243627268971924No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19110.3071641085731094No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18920.3041101483099545No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT17890.28755446898864084No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC17630.28337536547063935No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17170.2759815669387906No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC16040.25781854011055333No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG14750.2370837572712383No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA14740.23692302252054592No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA14640.23531567501362227No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC14480.23274391900254443No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA14230.2287255502352353No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCGACTTACAAGGCT14030.225510855221388No Hit
TGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG13380.2150630964263843No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13220.21249134041530643No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13060.20991958440422862No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA12710.20429386812999584No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGTTACTCCCTTAATTTCAT12390.19915035610784015No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11790.18950627106629825No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11750.18886333206352882No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT11350.1824339420358342No Hit
CTATCAATAATATCCTCTTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG11210.18018365552614107No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10830.17407573499983123No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG10760.17295059174498467No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10760.17295059174498467No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTCGGCGA10640.1710217747366763No Hit
GACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACAT10380.16684267121867483No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10280.16523532371175118No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG10150.16314577195275043No Hit
CTCTCGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA10100.16234209819928858No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT9820.15784152517990238No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9780.15719858617713292No Hit
CAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG9770.15703785142644056No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG9640.1549482996674398No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATA9420.15141213515220778No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9360.1504477266480536No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT9150.14707229688351392No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8950.14385760186966662No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8890.14289319336551243No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA8760.14080364160651168No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8750.14064290685581932No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC8430.13549939483366366No Hit
TAATAAAACCAAGCGTTGGGGTTACTCCCTTAATTTCATGGGATATGTAA8400.13501719058158654No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8310.13357057782535528No Hit
CATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGCAGCA8310.13357057782535528No Hit
CTATTAATAATATCCTCTTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG8190.1316417608170469No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT8160.1311595565649698No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8120.13051661756220032No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8030.12907000480596903No Hit
CAGTTCATCATTACTTCAAAACTAGGCATTATTTACATACTCTGTGGAAG7940.12762339204973777No Hit
TCACCATACAGCGCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA7880.12665898354558355No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAACGTAAGA7620.12247988002758209No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7610.12231914527688972No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7610.12231914527688972No Hit
GTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG7500.12055106301927371No Hit
ATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC7470.12006885876719661No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7410.11910445026304241No Hit
GTCCGAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7410.11910445026304241No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7360.1183007765095806No Hit
CTACCGTCCCCTTCTTTCTCTGCCGTTCCGGCCGACCACGGGGCGCACCT7230.11621122475057985No Hit
GATGAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7190.11556828574781038No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA7180.11540755099711801No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG7170.11524681624642567No Hit
AACATATTGTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAAT7160.11508608149573331No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7140.11476461199434856No Hit
AATTAAGGGAGTAACCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA7080.11380020349019437No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7050.11331799923811729No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCGCTCAGGCA6970.11203212123257836No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCGA6920.11122844747911655No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGT6880.11058550847634707No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6770.10881742621873106No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6760.10865669146803869No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAAT6740.10833522196665396No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6720.10801375246526924No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6670.10721007871180742No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTGAGTACAATA6640.10672787445973031No Hit
CCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6590.1059242007062685No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6560.1054419964541914No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6490.10431685319934486No Hit
ATTTTGTGGTCTGTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6380.10254877094172882No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6320.10158436243757464No Hit
GACCTGCTGCGAGCAAAACAAGCCGCTAGGAGTTCCGCAGTATGGATCGG6250.1004592191827281No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6240.10029848443203573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)