FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435550
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG26880.6171507289633796No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT25200.5785788084031684No Hit
GTTTATAAGAATACCAGTCATCTTGGATTGAAGCCCACCTTCATTTTAAC22200.5097003788313627No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT20520.4711284582711514No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18520.42520950522328094No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14170.32533578234416255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13390.30742739065549307No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13130.30145792675926986No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13130.30145792675926986No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9420.21627826885547008No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9060.20801285730685343No Hit
CTTATAAACAGGTGAAATTTGGAGACAGATAGACATGCAGCCAGAGGGGA8980.20617609918493857No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8810.2022729881758696No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8730.20043623005395478No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8570.19676271381012514No Hit
CTTCTGTATTGTTGTTTACTTATTAGGACCCCACATTATGGATTAAATTG8390.19263000803581679No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8300.1905636551486626No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8270.18987487085294458No Hit
GTGCTGGTGGCTGGGCAGAGAAAAATGGCAGGAGGGACAGAGTAAAAGCA8210.18849730226150843No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8070.18528297554815748No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8050.1848237860176788No Hit
CAGTAAAGGCTGTGAAGATGGAGAGAAAAAAATGGAGGGAAATTTGAGAA8030.1843645964872001No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8020.18413500172196073No Hit
ATACTGCGGAACTCCTAGCAGCGTGTTTTGCTCGCAGCCGGTCTGGGGCG7810.17931351165193435No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7760.17816553782573757No Hit
CTAATAATGTGACCTTATTTGGAGATAAGGTCCTTCCAGTTAAAATGAAG7740.17770634829525886No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7640.17541040064286534No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7300.16760417862472735No Hit
ACCATGGACTGGGTGGCTTAAACTGCAGAAATTCACCATTTCACAGTTCT7290.167374583859488No Hit
TATTAGGACCCCACATTATGGATTAAATTGTCCTCCCCAAATTCATATGT7270.1669153943290093No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7190.16507863620709448No Hit
CATTATTAGGGACGGGGAATTAGGACTTCAACATATGAATTTGGGGAGGA7180.1648490414418551No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6960.15979795660658935No Hit
GTATTGTTGTTTACTTATTAGGACCCCACATTATGGATTAAATTGTCCTC6820.15658362989323843No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA6750.15497646653656297No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6750.15497646653656297No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6570.15084376076225464No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6370.14625186545746757No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6060.13913442773504764No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6010.1379864539088509No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6010.1379864539088509No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5970.13706807484789346No Hit
CCGCCGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGA5680.13040982665595224No Hit
GTCTATCTGTCTCCAAATTTCACCTGTTTATAAGAATACCAGTCATCTTG5670.1301802318907129No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5540.12719549994260132No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5540.12719549994260132No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATATGTT5510.12650671564688326No Hit
GGATTAAATTGTCCTCCCCAAATTCATATGTTGAAGTCCTAATTCCCCGT5470.12558833658592583No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5440.12489955229020779No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5390.12375157846401103No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5350.12283319940305361No Hit
CTGTATTGTTGTTTACTTATTAGGACCCCACATTATGGATTAAATTGTCC5160.11847089886350591No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5150.11824130409826657No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATT5110.11732292503730914No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4990.11456778785443691No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4960.11387900355871886No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4920.11296062449776145No Hit
CTTATTAGGACCCCACATTATGGATTAAATTGTCCTCCCCAAATTCATAT4900.11250143496728275No Hit
CCCTAATAATGTGACCTTATTTGGAGATAAGGTCCTTCCAGTTAAAATGA4900.11250143496728275No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4870.11181265067156469No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4860.11158305590632533No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4830.11089427161060728No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAAT4820.11066467684536793No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCATTACAATA4810.11043508208012857No Hit
GAGTAAAAGCAGAAAGACAAAGGCAGAGCTAGGGCAGGAGCTGGGGAGGT4800.11020548731488923No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4710.10813913442773504No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4670.10722075536677762No Hit
TGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTAC4640.10653197107105958No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4630.10630237630582023No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4590.10538399724486282No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4500.10331764435770865No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4480.10285845482722994No Hit
AGACTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4470.10262886006199058No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4470.10262886006199058No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG4460.10239926529675124No Hit
ATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCACATTTCC4440.10194007576627252No Hit
GTTGTTTACTTATTAGGACCCCACATTATGGATTAAATTGTCCTCCCCAA4400.10102169670531513No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4380.10056250717483642No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4370.10033291240959706No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4370.10033291240959706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)