FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521287
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33820.6487788876377121No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21970.4214568941868875No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA18530.35546637456909536No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18380.35258888098111024No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16980.32573227415991574No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAGCCTTAT16180.31038564169066174No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15640.3000266647739153No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12390.23768097036757102No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC11640.22329350242764542No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11110.21312635841676467No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10990.21082436354637654No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTG10900.2090978673935855No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGC10090.19355940201846583No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10070.1931757362067345No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9750.1870370832190329No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9550.18320042510171938No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9170.17591077467882377No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9150.17552710886709239No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC8780.16842929135006243No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8140.15615198537465927No Hit
ATGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCC8050.1544254892218682No Hit
TAATAAGGCTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGG7990.15327449178667413No Hit
GGATAATAAGGCTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT7800.14962966657522633No Hit
ATTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTGCAGG7730.14828683623416658No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7670.14713583879897255No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7630.14636850717550984No Hit
GGATAAAGGTATTAAGCCTTATTATCCTGAACATGCAGTTAATCATTACT7560.1450256768344501No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7410.142148183246465No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7380.14157268452886798No Hit
TATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAA7220.13850335803501718No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG7210.1383115251291515No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6970.13370753538837532No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGTATTAAGC6950.13332386957664394No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6920.13274837085904695No Hit
GTATTAAGCCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT6570.12603421915374832No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6560.12584238624788266No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6460.1239240571892259No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6200.11893640163671834No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6140.1177854042015243No Hit
GGAACATATTGTGCTGAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6050.11605890804873324No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6050.11605890804873324No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6000.11509974351940486No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5960.11433241189594215No Hit
AGCCAGGACAGATTGGAGGACAAGAGGTTGGTGAGTGATTGGAGGTTGGG5930.11375691317834513No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5930.11375691317834513No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTT5900.1131814144607481No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5830.11183858411968838No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5810.11145491830795705No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5800.11126308540209136No Hit
GTTGTCAATATGCCTTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5720.10972842215516597No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5630.10800192600237488No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5610.10761826019064355No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5600.10742642728477786No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGATACTCCCTTAACTTCAT5560.10665909566131516No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC5530.10608359694371813No Hit
GTTAAGGGAGTATCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATAC5490.10531626532025544No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5460.10474076660265841No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5410.10378160207333005No Hit
GTCCTGGCTATCGCTGGATGTGTCTGCGGCGTTTTATCATATTCCTCTTC5380.10320610335573302No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5370.10301427044986736No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5280.10128777429707628No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5220.10013677686188222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)