FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471790
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18140.3844931007439751No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15040.3187859005065813No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12120.2568939570571653No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT12060.25562220479450604No Hit
CTACTAAAACCAAACGTTGGGGATACTCCCTTAACTTCATGGGCTATGTA10450.22149685241314993No Hit
GGGACTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA9900.2098391233387736No Hit
CTCAACAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9810.20793149494478477No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9590.2032684033150342No Hit
CATTTGAACCCTACTAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT9170.1943661374764196No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAA8910.18885521100489625No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8590.18207253227071366No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA8570.18164861484982725No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG8470.1795290277453952No Hit
CCCTTAACTTCATGGGCTATGTAATTGGAAGTTGGGGTACTTTACCACAG8440.17889315161406558No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8420.17846923419317917No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8350.17698552322007674No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8290.1757137709574175No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7910.16765933996057567No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7380.15642552830708578No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7340.15557769346531297No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA7250.1536700650713241No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7100.15049068441467603No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6830.14476779923270947No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6710.142224294707391No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6670.14137645986561817No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG6620.14031666631340214No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6520.1381970792089701No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6500.1377731617880837No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAG6420.13607749210453804No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6270.13289811144788996No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT6240.13226223531656034No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6210.13162635918523072No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6190.1312024417643443No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCATGTATA6110.12950677208079867No Hit
TACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTA6050.12823501981813942No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA5990.12696326755548018No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5910.12526759787193456No Hit
CATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5900.12505563916149134No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC5830.1235719281883889No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5820.1233599694779457No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCC5690.12060450624218402No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5650.11975667140041121No Hit
GGCCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC5560.11784904300642235No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5550.11763708429597915No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5400.11445770363933105No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5380.11403378621844464No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC5380.11403378621844464No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTAGTAGGGTTCAAATGTATAC5250.11127832298268296No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5220.11064244685135335No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5200.11021852943046695No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5140.1089467771678077No Hit
GTTTTAGTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5110.1083109010364781No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5080.10767502490514848No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5060.10725110748426206No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5000.10597935522160284No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTAGTAGGGTTCAAATGTATA4990.10576739651115961No Hit
CAATTACATAGCCCATGAAGTTAAGGGAGTATCCCCAACGTTTGGTTTTA4980.10555543780071643No Hit
ATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4970.10534347909027322No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4930.1044956442485004No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4920.10428368553805718No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4890.10364780940672756No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG4870.10322389198584117No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4860.10301193327539794No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4800.10174018101273873No Hit
TCCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT4780.1013162635918523No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4750.10068038746052269No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG4750.10068038746052269No Hit
CAACAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAAT4740.1004684287500795No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA4720.10004451132919308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)