FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471790
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20760.44002628288009493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14890.31560651984993326No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT12900.2734267364717353No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12050.2554102460840628No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11580.24544818669323215No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT10830.2295512834099917No Hit
GGGACTTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA10510.22276860467580914No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA9860.2089912884970008No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAA9420.19966510523749975No Hit
CTACTAAAACCAAACGTTGGGGATACTCCCTTAACTTCATGGGCTATGTA9360.1983933529748405No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8980.19033892197799868No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8920.18906716971533946No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8870.18800737616312344No Hit
CTCAACAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8800.18652366519002098No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA8520.18058882129761122No Hit
CCCTTAACTTCATGGGCTATGTAATTGGAAGTTGGGGTACTTTACCACAG8040.17041480319633734No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA7710.16342016575171156No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7650.16214841348905232No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7650.16214841348905232No Hit
CATTTGAACCCTACTAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT7630.1617244960681659No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7450.1579092392801882No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG7380.15642552830708578No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7180.15218635409822168No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7080.15006676699378962No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC6880.1458275927849255No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC6690.1418003772865046No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA6580.13946883147162933No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG6530.1384090379194133No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6520.1381970792089701No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAG6480.13734924436719725No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6440.13650140952542444No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6390.13544161597320842No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6350.13459378113143558No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCATGTATA6300.13353398757921958No Hit
GTTTTAGTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6230.13205027660611712No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6140.1301426482121283No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5750.12187625850484327No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5750.12187625850484327No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5740.12166429979440005No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5650.11975667140041121No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5540.11742512558553593No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5510.11678924945420631No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5450.11551749719154708No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCC5400.11445770363933105No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5340.11318595137667183No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5340.11318595137667183No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5320.1127620339557854No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5290.1121261578244558No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5250.11127832298268296No Hit
TACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTA5220.11064244685135335No Hit
TCCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT5020.10640327264248924No Hit
CCTCCTTCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGG5000.10597935522160284No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4990.10576739651115961No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4980.10555543780071643No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4960.10513152037983No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTAGTAGGGTTCAAATGTATAC4920.10428368553805718No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4910.10407172682761398No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4900.10385976811717078No Hit
CATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4810.10195213972318193No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4760.10089234617096589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)