FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386841
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32300.8349683720184778No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14670.37922557329755635No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12110.31304851347194323No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11950.3089124472328424No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10920.2822865208186309No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10780.2786674628594177No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9880.2554020902644756No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9780.25281704886503753No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9130.23601427976869047No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8390.2168849734128492No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8200.211973394753917No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8170.21119788233408557No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGGCAGACT8050.20809583265475998No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7950.20551079125532196No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7680.1985311794768393No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7670.19827267533689552No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7580.1959461380774013No Hit
CTCTATGACAGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7250.18741550145925587No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7030.18172841038049226No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6970.18017738554082943No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT6530.16880320338330218No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6400.16544264956403276No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6370.16466713714420136No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTTACAGT6290.16259910402465094No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5880.15200043428695512No Hit
ATCCAATATCTAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC5680.14683035148807907No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTAGATATTGGATCTTC5650.14605483906824768No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5580.14424531008864108No Hit
CACTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG5570.14398680594869726No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5540.14321129352886589No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5460.14114326040931546No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5450.14088475626937164No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5350.13829971486993364No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5330.13778270659004604No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5040.1302860865316758No Hit
ATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTCACGGTGGTCTCCATGC5020.1297690782517882No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5020.1297690782517882No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5010.12951057411184438No Hit
GTCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4990.1289935658319568No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4970.1284765575520692No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4940.1277010451322378No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4910.1269255327124064No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGAACAACAGAGT4890.12640852443251877No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCCTTGATTTTCTGT4850.12537450787274357No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4850.12537450787274357No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4850.12537450787274357No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4670.12072143335375515No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4670.12072143335375515No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA4600.11891190437414854No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4550.11761938367442955No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4540.11736087953448575No Hit
GACATATCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG4490.11606835883476675No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4440.11477583813504773No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4390.11348331743532873No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4390.11348331743532873No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG4370.11296630915544113No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC4360.11270780501549732No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4340.11219079673560972No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4280.11063977189594691No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4260.11012276361605931No Hit
CTTACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT4260.11012276361605931No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4220.10908874705628412No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4190.1083132346364527No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4160.10753772221662129No Hit
TTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCT4150.1072792180766775No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4140.1070207139367337No Hit
GTGTAAACAATACCTGAACCTTTACCCTGTTGCCCGGCAACGGCCAGGTC4120.10650370565684608No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4100.1059866973769585No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4070.10521118495712709No Hit
TTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTT4060.10495268081718329No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4060.10495268081718329No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATCGGTGGGCGTTC4030.10417716839735189No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4020.10391866425740809No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT4010.10366016011746429No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4010.10366016011746429No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAATGT4000.10340165597752048No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3980.10288464769763288No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3980.10288464769763288No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT3950.10210913527780147No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTAAGTGGGCCTAC3880.10029960629819486No Hit
CCCTTATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATGGA3870.10004110215825106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)