FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418576
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA35140.8395130155575093No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC15870.3791426168724437No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG14910.35620771377240934No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT14300.34163449409426244No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13130.3136825809410955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12330.29457016169106687No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11430.2730686900347846No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA11410.2725908795535339No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10780.2575398493941363No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10090.24105538779098656No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10030.23962195634723446No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9440.2255265471503383No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAAC9420.2250487366690876No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9340.2231374947440847No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG9000.21501471656282253No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8870.21190894843469288No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8820.21071442223156606No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT8720.20832536982531247No Hit
ATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTCACGGTGGTCTCCATGC8440.20163602308780246No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8220.19638010779404455No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT8190.19566339207216848No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTATGGTTTCACATTTCTT7980.19064638201903597No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7920.1892129505752838No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACT7710.1841959405221513No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7660.1830014143190245No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7620.18204579335652307No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG7600.18156798287527234No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7560.1806123619127709No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7510.17941783570964412No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7300.1744008256565116No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCATA7180.17153396276900731No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTTTGCTGGGGGGAATTAATGA6990.1669947631971255No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTC6990.1669947631971255No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6960.16627804747524944No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA6840.16341118458774512No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6840.16341118458774512No Hit
CATTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA6730.1607832269408662No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGTTGGATCTTC6690.15982760597836473No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA6600.1576774588127365No Hit
GCCTAGCAGCCATGGAAACGACGTATATTTGCGGGAGAGAACAACAGAGT6560.15672183785023508No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6550.15648293260960972No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6460.15433278544398152No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6400.15289935400022936No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6360.1519437330377279No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6310.1507492068346011No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA6240.1490768701502236No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5920.14143190245021214No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5910.14119299720958678No Hit
GTATTAAACCCTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5850.13975956576583465No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5720.136653797637705No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATCAGAGGACAAACGGGCAAC5720.136653797637705No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5710.13641489239707963No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5680.13569817667520356No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5630.13450365047207674No Hit
GTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5480.13092007186269639No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5390.12876992469706816No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGATC5360.12805320897519207No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5350.1278143037345667No Hit
GTTCTGGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5300.1266197775314399No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA5290.12638087229081457No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5280.1261419670501892No Hit
CCATAATAGTTGCCTGAACTTCAGGCCCATATTAGTGTTGACATAACTGA5260.1256641565689385No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5250.12542525132831314No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5160.12327510416268492No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAACTGCACC5140.12279729368143419No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5100.12184167271893276No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGCAGATCTCGTA5080.12136386223768204No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5010.11969152555330455No Hit
GTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5010.11969152555330455No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCTTCAACCACC4990.11921371507205383No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGCAGATCTCGTACTGAAGGAAA4980.11897480983142847No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTCTATCC4980.11897480983142847No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4940.11801918886892704No Hit
CTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4920.11754137838767631No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4870.11634685218454952No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4860.11610794694392415No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAA4830.11539123122204809No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACACC4760.11371889453767059No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCATCCCGATACAG4750.11347998929704523No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG4750.11347998929704523No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4720.11276327357516915No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4700.11228546309391843No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4680.11180765261266772No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4630.11061312640954092No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4630.11061312640954092No Hit
TCCTACATCTACCAATCGCCAGTCAGGAAGGCAGCCTACCCCACTGTCTC4620.11037422116891556No Hit
GTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGTA4590.10965750544703948No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4590.10965750544703948No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4570.10917969496578876No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4550.10870188448453805No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4540.10846297924391271No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4510.10774626352203663No Hit
CTATTATGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTCCT4500.10750735828141127No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCT4490.10726845304078592No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT4470.10679064255953519No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4450.10631283207828447No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4370.1044015901532816No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCT4330.10344596919078017No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC4330.10344596919078017No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAACTGC4220.10081801154390123No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC4200.10034020106265051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)