FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445790
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG34560.775252921779313No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT30300.6796922317683214No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT27760.622714731151439No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT25980.5827856165459073No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22140.4966464030148725No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19490.43720137284371563No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA19390.43495816415801164No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC17110.3838130061239597No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT15870.3559972184212297No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA15700.3521837636555329No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT14650.3286300724556406No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG14240.319432916844254No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG13610.30530070212431865No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC13220.2965521882500729No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT13210.2963278673815025No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC13070.29318737522151683No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC12690.2846631822158415No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG12430.27883083963301103No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG12300.2759146683415958No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11850.2658202292559277No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT11750.26357702057022364No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11510.25819331972453397No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC11490.25774467798739314No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC11490.25774467798739314No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11460.25707171538168194No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG11240.2521366562731331No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC11170.2505664101931403No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT11110.24922048498171784No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA10930.24518270934745062No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG10590.2375557998160569No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10480.23508827026178244No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA10340.23194777810179681No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA10300.2310504946275152No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG10300.2310504946275152No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG10180.22835864420467036No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG10160.22791000246752957No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10040.22521815204468473No Hit
GATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCC9960.22342358509612148No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA9940.2229749433589807No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG9560.21445075035330538No Hit
GAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9220.20682384082191166No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATC9030.202561744319074No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA8970.20121581910765157No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG8820.19785100607909553No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA8750.19628075999910274No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8730.1958321182619619No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8730.1958321182619619No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT8710.1953834765248211No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8620.19336458870768747No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8580.19246730523340586No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC8480.19022409654770184No Hit
CTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG8370.18775656699342738No Hit
TTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTTCTAAAAC8370.18775656699342738No Hit
GGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT8360.187532246124857No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG8350.1873079252562866No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC8290.18596200004486418No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8290.18596200004486418No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA8120.18214854527916732No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT8110.1819242244105969No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8060.1808026200677449No Hit
TCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACGCTGGTGGTTG8050.1805782991991745No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC7960.17855941138204087No Hit
CCTGTTAACAGGCCAATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7940.17811076964490005No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCAT7820.17541891922205524No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7700.1727270687992104No Hit
GGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAA7640.17138114358778797No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT7290.16352991318782387No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7280.16330559231925346No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC7270.16308127145068305No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA7250.16263262971354225No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT7250.16263262971354225No Hit
GTCTAGATTGAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACAT7230.16218398797640143No Hit
ACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGATT7010.15724892886785258No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6930.15545436191928935No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG6910.15500572018214853No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAA6910.15500572018214853No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGC6840.15343547410215572No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6830.1532111532335853No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC6750.1514165862850221No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6670.14962201933645888No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA6670.14962201933645888No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6630.14872473586217727No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6630.14872473586217727No Hit
CCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATGGA6620.14850041499360686No Hit
ATACGTTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTTCT6580.14760313151932525No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAGAC6540.1467058480450436No Hit
CAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTG6540.1467058480450436No Hit
ATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTT6490.1455842437021916No Hit
GTGAAAGCCTGTCTAGATTGAATACAGGCATACAAGGGCATTAAAGCAGG6410.1437896767536284No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG6400.14356535588505798No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGAT6330.14199510980506516No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6230.13975190111936114No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6180.13863029677650912No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTC6170.1384059759079387No Hit
ACAATACGTTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTT6160.13818165503936833No Hit
AATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATA6160.13818165503936833No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTCT6130.1375086924336571No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6120.13728437156508672No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG6100.1368357298279459No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC6090.13661140895937549No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAGACAGGCTTTCACTTT6070.1361627672222347No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAG6070.1361627672222347No Hit
CATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGT6060.13593844635366428No Hit
GGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGC6030.13526548374795308No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA6020.13504116287938267No Hit
CTATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCA6000.13459252114224185No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5940.13324659593081944No Hit
GGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAACAGTAGT5920.13279795419367865No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTCTAGATTGAA5810.1303304246394042No Hit
CAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5780.129657462033693No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC5740.12876017855941138No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5730.12853585769084097No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5730.12853585769084097No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA5710.12808721595370015No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5700.12786289508512977No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATTGGCCTGT5690.12763857421655936No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5670.12718993247941857No Hit
TTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGG5630.12629264900513695No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT5600.12561968639942575No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5480.1229278359765809No Hit
CAATACGTTGACAGACTTTCCAATCAATTGGCCTGTTAACAGGAAGTTTT5470.1227035151080105No Hit
GTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAAT5420.12158191076515848No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5390.12090894815944726No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAT5360.12023598555373606No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5330.11956302294802487No Hit
CCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCG5310.11911438121088404No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTC5280.11844141860517285No Hit
GGTTCTGCATGGTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGAC5270.11821709773660244No Hit
CCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCG5230.11731981426232083No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG5220.11709549339375043No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5200.11664685165660961No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA5150.1155252473137576No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5140.1153009264451872No Hit
GGCCAATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT5140.1153009264451872No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT5130.11507660557661678No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5090.11417932210233518No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5050.11328203862805357No Hit
CCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGC5030.11283339689091276No Hit
GGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCA5020.11260907602234237No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC5010.11238475515377197No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5000.11216043428520155No Hit
GCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4980.11171179254806074No Hit
CCTGTATTCAATCTAGACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4980.11171179254806074No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC4960.11126315081091995No Hit
GCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTT4930.11059018820520873No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG4890.10969290473092713No Hit
CTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4890.10969290473092713No Hit
GTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCGAGGATTGGGGA4880.10946858386235671No Hit
GTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTC4850.1087956212566455No Hit
TCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTT4800.10767401691379348No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC4780.10722537517665269No Hit
ATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATGTATCCCTCCT4770.10700105430808228No Hit
TTGTATGCCTGTATTCAATCTAGACAGGCTTTCACTTTCTCGCCAACTTA4730.10610377083380067No Hit
GCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACC4720.10587944996523027No Hit
GTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACCAGCGTG4710.10565512909665986No Hit
GTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGG4680.10498216649094864No Hit
CCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATC4670.10475784562237825No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAGACAG4670.10475784562237825No Hit
TCGTAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGG4590.10296327867381502No Hit
GGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG4580.10273895780524463No Hit
TGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATGTATCCCTCCTG4530.1016173534623926No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT4520.10139303259382221No Hit
GGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACCAGCGT4480.10049574911954058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)