FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500349
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22180.4432905831729453No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA15620.31218209689636633No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAAT11090.22164529158647264No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8020.16028811889301267No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTATTACATAATCT7390.14769690755852416No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7130.14250053462683046No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6760.13510569622403562No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6500.12990932329234192No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC6400.1279107183186136No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6380.12751099732386795No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6100.1219149033974286No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATCATTTAAATTTCAT6070.1213153219053101No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5860.11711825146048058No Hit
GTGTAGACTGAGTGAAAAATAACCTTTCATAGTTTGAACGTACTATGTTT5750.11491978598937942No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5240.1047269006233649No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA5150.10292815614700938No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5150.10292815614700938No Hit
ATACAAACCTTGCCAGCAAATCCACCTCCTGCATCCACCAATCGCCAGTC5060.10112941167065388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)