FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500349
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85451.7078079500508645No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14470.28919813969849045No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCATCTTTTCCTAAT9750.19486398493851292No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7310.1460980235795415No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTATTACATAATCT7070.14130137164259346No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6590.13170806776869745No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6350.12691141583174942No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6340.1267115553343766No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATCATTTAAATTTCAT6190.12371364787378411No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6080.12151518240268293No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6030.12051587991581876No Hit
GTGTAGACTGAGTGAAAAATAACCTTTCATAGTTTGAACGTACTATGTTT5990.11971643792632744No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5630.11252146002090542No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC5500.10992327355505856No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5170.10332787714175506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)