FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468494
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35080.7487822682894552No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12970.2768445273578744No Hit
GTGCTGGTGTGTGCGGCGCCTGTTGGTGAACTATTGCAGAGCCTCCCAGC7340.15667223059420185No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA7240.15453773153978492No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6780.1447190358894671No Hit
GTGCTGGTGCCTGTAGTCCCAGCTACTCGGGAGGCGGAGGCAGGAGAATG6550.13980968806430819No Hit
GTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCATTCTCTGAAGTC6440.13746173910444956No Hit
TGTTATCACTGTGTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCA6420.1370348392935662No Hit
GTGCTGGTGGACGGGTCCAGGCCTGAGAAGCCGTAGTCTCCTAGTGCTTG5730.12230679581808944No Hit
ATATAGAATTGGGACTCAAAATATTCGTGGAGTGAATGACCAGATTAATC5370.11462259922218855No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5290.112914999978655No Hit
TCATTACACACAGTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCA5010.10693840262628765No Hit
GTTCATAGGCAGTTCACACACAGCTGTTTGCTTTTCTCTTCTCAGTGAAT4750.10138870508480365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)