FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468494
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93201.9893531187165685No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA12220.2608357844497475No Hit
GTGCTGGTGTGTGCGGCGCCTGTTGGTGAACTATTGCAGAGCCTCCCAGC7520.1605143288921523No Hit
TGTTATCACTGTGTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCA6810.14535938560579217No Hit
GTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCATTCTCTGAAGTC6540.1395962381588665No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA6430.1372482891990079No Hit
GTGCTGGTGCCTGTAGTCCCAGCTACTCGGGAGGCGGAGGCAGGAGAATG6390.1363944895772411No Hit
ATATAGAATTGGGACTCAAAATATTCGTGGAGTGAATGACCAGATTAATC5130.10949980149158793No Hit
TCATTACACACAGTGATAACATTAGCCAGAATATCCGTTTTTTGCACTCA5130.10949980149158793No Hit
GTGTAATGAATTGTCCTGTTTCTCTGAACCAAGAGTCATGTGTCTTCTGC4980.10629805290996257No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4950.10565770319363749No Hit
GTGCTGGTGGACGGGTCCAGGCCTGAGAAGCCGTAGTCTCCTAGTGCTTG4760.10160215499024534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)