FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452695
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA41580.9184992102850705No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT19830.4380432741691425No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT16780.3706689934724262No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14710.3249428423110483No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT14640.3233965473442384No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT13110.2895989573553938No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12790.2825301803642629No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12260.2708225184727024No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11580.25580136736654924No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11490.2538132738377937No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10920.24122201482234176No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10710.2365831299219121No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT10550.23304874142634663No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC10270.22686356155910714No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT10090.22288737450159601No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10090.22288737450159601No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9710.21449320182462808No Hit
CACTAATATGGGCTTAAAATTCAGGCAACTTCTGTGGTTTCACATTTCTT9620.21250510829587252No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9270.2047736334618231No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9200.20322733849501318No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9190.20300643921404038No Hit
AGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT9150.20212284209014897No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8160.1802538132738378No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8140.1798120147118921No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC8100.17892841758800077No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7780.17185964059686984No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGGGAGAGAACAACAGAGT7610.16810435282033157No Hit
CTCACAGTCAATGAAAAACGAAGACTGAAATTAATTATGCCTGCTAGGTT7520.166116259291576No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7320.1616982736721192No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7220.15948928086239078No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7180.15860568373849943No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7040.15551309380487965No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA7030.15529219452390683No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6980.15418769811904262No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6980.15418769811904262No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6970.15396679883806977No Hit
GGGTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT6940.15330410099515127No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTC6900.15242050387125988No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6880.1519787053093142No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC6850.1513160074663957No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6800.1502115110615315No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6700.14800251825180308No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCGTTTTTCATTGAC6590.14557262616110184No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6450.14248003622748207No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6240.13784115132705244No Hit
CTTTTACACAATGTGGTTATCCTGCTTTGATGCCCTTGTATGCCTGTATT6210.1371784534841339No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6160.1360739570792697No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6140.13563215851732402No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTG6080.134306762831487No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCGTTTTTCA6070.13408586355051413No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6020.13298136714564993No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6020.13298136714564993No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6020.13298136714564993No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5910.13055147505494868No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5810.1283424822452203No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5800.12812158296424744No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5790.12790068368327462No Hit
GGAAAACACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAA5790.12790068368327462No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5710.12613348943549188No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5670.12524989231160052No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5630.12436629518770917No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5590.12348269806381779No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5570.12304089950187212No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA5550.12259910093992644No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5540.1223782016589536No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5520.12193640309700793No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5490.1212737052540894No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5470.12083190669214372No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5440.1201692088492252No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5390.119064712444361No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5380.11884381316338816No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5350.11818111532046964No Hit
CTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTGTACCTGTC5340.1179602160394968No Hit
GTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTG5240.11575122322976837No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5230.11553032394879553No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC5230.11553032394879553No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5220.11530942466782271No Hit
ACATAGAGGTTCCTTGAACAGTAGTCGTGCAGGTTCTGCATGGTCCCACG5140.11354223042003997No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5140.11354223042003997No Hit
GGACAACACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAA5130.11332133113906714No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5120.1131004318580943No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5100.11265863329614863No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5080.11221683473420295No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5070.11199593545323011No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5020.1108914390483659No Hit
ACCCTTCACGGGGCCGTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG5000.11044964048642023No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5000.11044964048642023No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4950.10934514408155602No Hit
GAATAAAGCCCTGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC4930.10890334551961034No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4920.1086824462386375No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4820.1064734534289091No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4810.10625255414793626No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4750.10492715846209921No Hit
TTGTAGGCCCACTCACAGTCAATGAAAAACGAAGACTGAAATTAATTATG4740.10470625918112637No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4710.10404356133820783No Hit
GTTATGGGCCATCAGCGCATGCGTGGAACCTTTCTGGCTCCTCTGCCGAT4620.10205546780945227No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4580.10117187068556092No Hit
GTAAAAGCCTGCCTAGATTGAATACAGGCATACAAGGGCATCAAAGCAGG4550.10050917284264241No Hit
GTTCTGGGTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA4550.10050917284264241No Hit
ACACTAATATGGGCTTAAAATTCAGGCAACTTCTGTGGTTTCACATTTCT4540.10028827356166957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)