FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452695
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA38700.8548802173648925No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16860.372436187720209No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT14360.31721136747699885No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14160.312793381857542No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT13780.3043992091805741No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13350.29490054009874195No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12850.2838555760500999No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12560.2774494969018876No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12240.2703807199107567No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC11980.26463733860546285No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11710.25867305801919616No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT11630.2569058637714134No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT11380.2513833817470924No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11050.24409370547498868No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10690.23614133135996643No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9830.21714399319630212No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9150.20212284209014897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9130.2016810435282033No Hit
CACTAATATGGGCTTAAAATTCAGGCAACTTCTGTGGTTTCACATTTCTT8870.19593766222290948No Hit
AGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT8690.19196147516539835No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8670.19151967660345265No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA8230.18180010824064766No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC8180.18069561183578348No Hit
GGGTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTT8060.17804482046410938No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8040.1776030219021637No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7720.17053424491103283No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC7580.16744165497741303No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7350.1623609715150377No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7320.1616982736721192No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT7290.1610355758292007No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCGTTTTTCATTGAC7190.15882658301947228No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7100.15683848949071671No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCGTTTTTCA7050.1557339930858525No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7020.155071295242934No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGGGAGAGAACAACAGAGT7010.15485039596196115No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7000.1546294966809883No Hit
CTTTTACACAATGTGGTTATCCTGCTTTGATGCCCTTGTATGCCTGTATT6900.15242050387125988No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6750.1491070146566673No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6640.14667712256596604No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG6540.14446812975623763No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6530.1442472304752648No Hit
GTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG6510.14380543191331913No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6500.14358453263234627No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6460.14270093550845492No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6350.14027104341775368No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6330.139829244855808No Hit
ACCCTTCACGGGGCCGTTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG6310.13938744629386232No Hit
ATATTAGTGTTGACATAACTAACAACTAGGTCCCTAGATGCTGGATCTTC6310.13938744629386232No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6250.13806205060802526No Hit
GTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTG6250.13806205060802526No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6180.13651575564121537No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6180.13651575564121537No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6080.134306762831487No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6030.13320226642662278No Hit
CTCACAGTCAATGAAAAACGAAGACTGAAATTAATTATGCCTGCTAGGTT5930.13099327361689436No Hit
GTTCTGGGTAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5910.13055147505494868No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5880.12988877721203018No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA5850.12922607936911165No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5850.12922607936911165No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5840.1290051800881388No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5820.12856338152619312No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5730.12657528799743756No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTG5610.12392449662576349No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5540.1223782016589536No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5390.119064712444361No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5390.119064712444361No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5350.11818111532046964No Hit
CTCTAAGGCTTCCCGATAGAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5310.11729751819657827No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5290.1168557196346326No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5220.11530942466782271No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5190.11464672682490419No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5120.1131004318580943No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5070.11199593545323011No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4980.11000784192447452No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT4970.10978694264350168No Hit
GGAAAACACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAA4950.10934514408155602No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4900.10824064767669182No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4890.10801974839571896No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4880.10779884911474612No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4860.10735705055280045No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA4850.10713615127182761No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4790.10581075558599055No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4740.10470625918112637No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC4740.10470625918112637No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4730.10448535990015353No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC4690.10360176277626215No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4660.10293906493334365No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4620.10205546780945227No Hit
TTGTAGGCCCACTCACAGTCAATGAAAAACGAAGACTGAAATTAATTATG4610.10183456852847943No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4600.10161366924750659No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4590.10139276996653376No Hit
GGACAACACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAA4590.10139276996653376No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA4580.10117187068556092No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4580.10117187068556092No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4580.10117187068556092No Hit
CTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTGTACCTGTC4560.10073007212361525No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4540.10028827356166957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)