FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415941
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA26280.6318203783709708No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT14560.3500496464642822No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12530.3012446476783967No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10390.2497950430469706No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10290.24739085591466095No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9830.23633159510603666No Hit
CATTTAAACCCTAATAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9460.22743610271649103No Hit
CATTTACACCAAAACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9100.21878102904017635No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9060.21781935418725254No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9010.21661726062109768No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT8730.20988553665063073No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8570.20603883723893532No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8300.19954753198169933No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8280.1990666945552374No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8200.1971433448493897No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8180.19666250742292776No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACGACACATAGCGCC8110.19497957643031102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8010.19257538929800141No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC7900.1899307834524608No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7890.18969036473922984No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA7830.18824785245984405No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7680.18464157176137963No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7190.1728610548130624No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7030.16901435540136703No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6870.16516765598967162No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6590.15843593201920464No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGATGGAAAGAAGT6510.15651258231335693No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT6390.1536275577545854No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6310.1517042080487377No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6240.15002127705612095No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6210.14930002091642805No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6160.14809792735027322No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6060.1456937402179636No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5830.14016410981365143No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5820.1399236911004205No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5810.13968327238718953No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5810.13968327238718953No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5650.13583657297549412No Hit
AATATACATTTACACCAAAACATTATCAAAAAATGTGAACAATTTGTAGG5640.13559615426226315No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5630.13535573554903219No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5610.13487489812257028No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5520.13271112970349158No Hit
GCCCTAAAGGTTCCACGCATACGCTGATGGCCCATGACCAAGCCCCAGCC5490.1319898735637987No Hit
AATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTTGTCT5460.1312686174241058No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5450.13102819871087487No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC5430.13054736128441294No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCATCAGTACG5350.12862401157856523No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA5350.12862401157856523No Hit
CCTAAAACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5350.12862401157856523No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5350.12862401157856523No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5320.12790275543887233No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5140.123575218600715No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5100.12261354374779115No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5060.1216518688948673No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5010.12044977532871248No Hit
ATATAAGAGAGAAACGACACATAGCGCCTCATTTTGTGGGTCACCATATT5010.12044977532871248No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4960.11924768176255766No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4950.11900726304932671No Hit
CCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC4940.11876684433609573No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCGCACCA4870.117083913343479No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4870.117083913343479No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4820.11588181977732419No Hit
ATCTAGAAGATCTCGTACTGATGGAAAGAAGTCAGTAGGCAAAAACGAGA4820.11588181977732419No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4820.11588181977732419No Hit
GTTATGTTAACACTAATGTGGGCCTAAAGTTTAGGCAACTATTGTGGTTT4770.11467972621116937No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4740.11395847007147647No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4690.11275637650532168No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4680.1125159577920907No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4590.11035218937301204No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4550.10939051452008818No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4550.10939051452008818No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4510.10842883966716434No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4500.10818842095393338No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4460.10722674610100952No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4450.10698632738777855No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4430.10650548996131662No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC4420.10626507124808567No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4420.10626507124808567No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4400.10578423382162373No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4400.10578423382162373No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4370.10506297768193085No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG4320.10386088411577603No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCCTCGGACGGAAATTGCACC4270.10265879054962121No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4260.10241837183639026No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4250.1021779531231593No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4250.1021779531231593No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4200.10097585955700449No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA4190.10073544084377353No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATGTGGGC4190.10073544084377353No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACAAC4180.10049502213054255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)