FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415941
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24590.5911896158349381No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT15490.37240858679476174No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12270.2949937611343917No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11580.2784048699214552No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10630.2555650921645137No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10040.24138038808388687No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9370.2252723342974124No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC9040.21733851676079058No Hit
CATTTAAACCCTAATAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9010.21661726062109768No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8940.21493432962848097No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8830.21228972378294036No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACGACACATAGCGCC8800.21156846764324747No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8550.2055579998124734No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC8490.20411548753308761No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8410.20219213782723994No Hit
CATTTACACCAAAACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8090.19449873900384912No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8080.19425832029061813No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7760.18656492146722733No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7730.18584366532753444No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7730.18584366532753444No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7440.1788715226438365No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7420.17839068521737458No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7070.16997603025429087No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6980.16781226183521222No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6920.16636974955582642No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6830.16420598113674775No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6770.16276346885736198No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6740.16204221271766908No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6530.15699341973981887No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6310.1517042080487377No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6280.1509829519090448No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6210.14930002091642805No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6160.14809792735027322No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT6150.14785750863704228No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5840.14040452852688243No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA5710.1372790852548799No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5660.13607699168872509No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5580.13415364198287738No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5520.13271112970349158No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA5380.1293452677182581No Hit
GCCCTAAAGGTTCCACGCATACGCTGATGGCCCATGACCAAGCCCCAGCC5340.12838359286533427No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGATGGAAAGAAGT5310.12766233672564137No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5310.12766233672564137No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC5190.12477731216686981No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5170.1242964747404079No Hit
CCTAAAACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5160.12405605602717693No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5150.12381563731394597No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC5110.12285396246102212No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5030.12093061275517442No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4970.11948810047578864No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC4960.11924768176255766No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4920.11828600690963383No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCGCACCA4890.11756475076994094No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4880.11732433205670996No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4810.11564140106409324No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4800.11540098235086226No Hit
CCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC4800.11540098235086226No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA4780.11492014492440034No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4780.11492014492440034No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4780.11492014492440034No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4750.11419888878470745No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4740.11395847007147647No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4730.11371805135824552No Hit
AATATACATTTACACCAAAACATTATCAAAAAATGTGAACAATTTGTAGG4730.11371805135824552No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCATCAGTACG4670.11227553907885975No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG4620.11107344551270493No Hit
AATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTTGTCT4600.110592608086243No Hit
ATATAAGAGAGAAACGACACATAGCGCCTCATTTTGTGGGTCACCATATT4550.10939051452008818No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4530.10890967709362627No Hit
CTATGTATCCCTCTTGTTGCTGTACCAAACCCTCGGACGGAAATTGCACC4490.1079480022407024No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACAAC4480.10770758352747145No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4460.10722674610100952No Hit
CTTTAGGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT4450.10698632738777855No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4440.1067459086745476No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4440.1067459086745476No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC4430.10650548996131662No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4380.1053033963951618No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4350.10458214025546891No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4340.10434172154223796No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG4340.10434172154223796No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4340.10434172154223796No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC4320.10386088411577603No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA4300.10338004668931412No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT4270.10265879054962121No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA4270.10265879054962121No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4250.1021779531231593No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA4240.10193753440992832No Hit
ATCTAGAAGATCTCGTACTGATGGAAAGAAGTCAGTAGGCAAAAACGAGA4230.10169711569669737No Hit
GTTATGTTAACACTAATGTGGGCCTAAAGTTTAGGCAACTATTGTGGTTT4210.10121627827023544No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT4210.10121627827023544No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4190.10073544084377353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)