FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540134
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22650.4193403859042386No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCATAGTTT18560.3436184354252833No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG17430.32269770094087763No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAAGGAGTTTG16750.31010823240158925No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15230.28196706743141514No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15150.2804859534856165No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA14270.2641937000818316No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14150.2619720291631336No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11820.21883458549174836No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11710.2167980538162752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11650.21568721835692625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10790.19976524343959093No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10690.19791385100734263No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10430.19310023068349708No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10410.1927299521970474No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10370.19198939522414807No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10280.19032314203512463No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA10240.1895825850622253No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCATAGT10100.1869906356570777No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTAGCAATGTCAACGACCGACCT10040.1858798001977287No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA9930.18384326852225558No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTAAC9930.18384326852225558No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9920.18365812927903075No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAGAGTCCAA9630.1782890912255107No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9570.17717825576616172No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9390.1738457493881148No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9370.17347547090166515No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9200.17032810376684304No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC9140.16921726830749406No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8960.16588476192944712No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCTTTTC8750.16199683782172572No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8740.1618116985785009No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTAACATTCATTTACAGG8700.16107114160560157No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8490.15718321749788017No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8280.15329529339015874No Hit
GTAGATGCCCAGACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG8110.15014792625533666No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8090.14977764776888697No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7720.14292749576956829No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG7360.13626248301347443No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7300.13515164755412548No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG7280.1347813690676758No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7130.13200428041930334No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6980.12922719177093092No Hit
GGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGC6960.12885691328448126No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6880.12737579933868262No Hit
GAGTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCA6840.1266352423657833No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT6820.12626496387933364No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6780.12552440690643432No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA6780.12552440690643432No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6650.12311759674451155No Hit
ATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG6600.1221919005283874No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6570.1216364827987129No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6520.12071078658258876No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6430.1190445333935653No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6410.11867425490711564No Hit
CCTTTATACGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC6350.11756341944776666No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6330.117193140961317No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA6330.117193140961317No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6200.11478633079939422No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6170.11423091306971975No Hit
TATTAACATTGACATAACTGACTACTAATTCCCTGGATGCTGGGTCTTCC6160.1140457738264949No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6150.11386063458327007No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA6120.11330521685359558No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6110.11312007761037075No Hit
TCCACAGAGTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCA6050.11200924215102179No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6000.11108354593489762No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5960.11034298896199832No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5910.10941729274587418No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5870.10867673577297486No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA5850.10830645728652519No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5790.10719562182717622No Hit
CCTGTAAATGAATGTTAGGAAAAGAAGGAGTTTGCCACTCAGGATTAAAG5780.10701048258395139No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTT5680.1051590901517031No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5660.10478881166525343No Hit
CATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGA5640.10441853317880379No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGT5610.1038631154491293No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5590.10349283696267962No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5580.1033076977194548No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5560.10293741923300515No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5520.10219686226010583No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5480.1014563052872065No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA5410.1001603305846327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)