FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540134
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCAGG27650.5119100075166533No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCATAGTTT17820.32991813142664594No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAAGGAGTTTG17260.3195503338060555No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG16720.30955281467191476No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14300.26474911781150606No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13850.2564178518663887No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA13660.25290020624511694No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13630.25234478851544245No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13360.24734602894837207No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTAGCAATGTCAACGACCGACCT12020.22253737035624493No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11640.2155020791137014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11580.21439124365435244No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11500.2129101297085538No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11470.21235471197887928No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA11380.21068845878985587No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11130.20605997770923512No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10990.2034680283040875No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCATAGT10870.20124635738538954No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10830.20050580041249022No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10280.19032314203512463No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10130.18754605338675218No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA10100.1869906356570777No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC9990.18495410398160456No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAGAGTCCAA9470.17532686333391342No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9390.1738457493881148No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCTTTTC9020.1669955973887961No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTAAC8910.16495906571332297No Hit
GGGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGC8570.1586643314436788No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8410.15570210355208153No Hit
CTTTAATCCTGAGTGGCAAACTCCTTCTTTTCCTAACATTCATTTACAGG8370.1549615465791822No Hit
GTATACATGCGTATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8330.1542209896062829No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8030.14866681230953802No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG7930.14681541987728972No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7850.14533430593149108No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7810.14459374895859176No Hit
GTAGATGCCCAGACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG7800.14440860971536693No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7630.14126124258054484No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATA7630.14126124258054484No Hit
GAGTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCA7440.13774359695927307No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7240.13404081209477647No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7040.13033802723027987No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6950.12867177404125643No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6930.12830149555480677No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6900.12774607782513228No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATACGCATGTATA6880.12737579933868262No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6860.12700552085223296No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6840.1266352423657833No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6830.12645010312255847No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG6830.12645010312255847No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6740.12478384993353503No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6730.12459871069031019No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6600.1221919005283874No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6400.11848911566389082No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT6350.11756341944776666No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6310.11682286247486735No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6190.1146011915561694No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6180.11441605231294458No Hit
GTTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTT6160.1140457738264949No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6130.11349035609682043No Hit
CCTTTATACGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC6080.11256465988069626No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6050.11200924215102179No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5980.11071326744844798No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5980.11071326744844798No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5980.11071326744844798No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5950.1101578497187735No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5920.109602431989099No Hit
TCCACAGAGTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCA5910.10941729274587418No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5870.10867673577297486No Hit
ATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG5770.10682534334072655No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5700.10552936863815275No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5650.10460367242202862No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAAT5630.10423339393557894No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5580.1033076977194548No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTATGCATTATT5560.10293741923300515No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA5510.10201172301688098No Hit
GTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCCTG5480.1014563052872065No Hit
CCTGTAAATGAATGTTAGGAAAAGAAGGAGTTTGCCACTCAGGATTAAAG5480.1014563052872065No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTT5480.1014563052872065No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5470.10127116604398169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)