FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325930
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCTTGTTCAGG16190.49673242720829625No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT11530.3537569416745927No Hit
CTTATATAGAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTCT10840.33258675175651214No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC10580.32460957874390206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9350.28687141410732364No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9030.2770533550148805No Hit
ATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8630.2647807811493265No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8480.2601785659497438No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8380.2571104224833553No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8210.2518945785904949No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA7990.2451446629644402No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7660.23501978952535824No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7460.22888350259258122No Hit
CTCTTATATAGAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7260.22274721565980427No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGATAGGACAACAGAGT7190.22059951523333232No Hit
GTCTTGAAGTAATGATTAACTGCTTGTTCAGGATAATATGGTTTAATGCC7190.22059951523333232No Hit
GTGTAAAAGGGGCGGCAAAGCCCAACAGACCCACAATTCTCTGACATACT7190.22059951523333232No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7130.21875862915349925No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7090.21753137176694382No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6720.2061792409413064No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6590.2021906544350014No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6490.1991225109686129No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6420.19697481054214094No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6160.1889976375295309No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5840.1791795784370877No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5350.16414567545178413No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5260.16138434633203447No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT4950.15187310158623016No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC4940.1515662872395913No Hit
TTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4920.15095265854631362No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATGTCCTCCTGT4870.14941858681311937No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4850.14880495811984168No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT4840.14849814377320283No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4840.14849814377320283No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4740.14543000030681436No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4710.1445095572668978No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4710.1445095572668978No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT4690.1438959285736201No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4600.14113459945387047No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4570.1402141564139539No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4560.13990734206731506No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4550.13960052772067622No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4450.13653238425428774No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4420.13561194121437117No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4400.13499831252109348No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4360.1337710551345381No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4300.131930169054705No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4240.1300892829748719No Hit
GAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCTTGTTCA4230.12978246862823306No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4230.12978246862823306No Hit
ATATTATCCTGAACAAGCAGTTAATCATTACTTCAAGACTAGGCATTATT4190.12855521124167765No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4190.12855521124167765No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4160.12763476820176112No Hit
GTTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCC4110.12610069646856686No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4110.12610069646856686No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4100.12579388212192802No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4100.12579388212192802No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTCTATATAAGAGAGAAACTACA4070.12487343908201148No Hit
GTTGTCAATAGACCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4050.12425981038873377No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4020.12333936734881723No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4020.12333936734881723No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4010.12303255300217839No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4000.12272573865553954No Hit
CAATAGACCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG3980.12211210996226185No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3950.12119166692234529No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3940.12088485257570644No Hit
ATACAGCACTCAGGCAAGCTATCCTGTGTTGGGGTGAGTTGATGAATCTG3930.1205780382290676No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3920.12027122388242875No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3920.12027122388242875No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3850.11812352345595681No Hit
TCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTT3820.11720308041604025No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC3820.11720308041604025No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3790.11628263737612371No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3790.11628263737612371No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3780.11597582302948486No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3750.1150553799895683No Hit
GTTCAGGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA3710.11382812260301292No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC3700.11352130825637408No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3700.11352130825637408No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT3680.11290767956309639No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC3670.11260086521645751No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3670.11260086521645751No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3660.11229405086981867No Hit
TCCACAGAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCT3630.11137360782990213No Hit
CTCCTCGACACCGCCTCAGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA3620.11106679348326329No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3610.11075997913662444No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3560.10922590740343019No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG3540.1086122787101525No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3530.10830546436351365No Hit
GTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCCTG3520.10799865001687478No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3520.10799865001687478No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC3500.10738502132359709No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3460.10615776393704171No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3440.10554413524376399No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3440.10554413524376399No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC3430.10523732089712515No Hit
CTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG3430.10523732089712515No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3420.1049305065504863No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3400.10431687785720861No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3370.10339643481729205No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG3350.10278280612401436No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT3350.10278280612401436No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3340.10247599177737551No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3310.10155554873745896No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3300.10124873439082012No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3300.10124873439082012No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA3300.10124873439082012No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT3280.10063510569754242No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3270.10032829135090357No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3270.10032829135090357No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3260.10002147700426472No Hit
CTTCAAGACTAGGCATTATTTACATACTCTGTGGAAGGCGGGTATTCTAT3260.10002147700426472No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3260.10002147700426472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)