FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325930
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCTTGTTCAGG17780.5455159083238732No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC11070.33964348172920567No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCATAT10840.33258675175651214No Hit
CTTATATAGAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTCT9970.30589390359893226No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9840.30190531709262725No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8940.27429202589513085No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8520.2614058233362992No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8470.25987175160310494No Hit
ATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG8220.25220139293713373No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7800.23931519037830207No Hit
GTCTTGAAGTAATGATTAACTGCTTGTTCAGGATAATATGGTTTAATGCC7780.23870156168502438No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA7750.23778111864510787No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7730.23716748995183018No Hit
GTGTAAAAGGGGCGGCAAAGCCCAACAGACCCACAATTCTCTGACATACT7730.23716748995183018No Hit
CTCTTATATAGAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7630.23409934648544167No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7250.2224404013131654No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7100.2178381861135827No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA7070.21691774307366612No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6950.21323597091399993No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6790.20832694136777835No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6630.20341791182155677No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6500.19942932531525176No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6470.1985088822753352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6130.18807719448961432No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGATAGGACAACAGAGT6070.18623630840978123No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5580.17120240542447765No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5340.16383886110514526No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5310.16291841806522872No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5120.1570889454790906No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5080.1558616880925352No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5040.15463443070597982No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5030.15432761635934095No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT5000.1534071733194244No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT4980.15279354462614672No Hit
GAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCTTGTTCA4890.1500322155063971No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4810.14757770073328627No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC4720.14481637161353664No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4690.1438959285736201No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4540.13929371337403737No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4520.13868008468075968No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4520.13868008468075968No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT4460.13683919860092658No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4450.13653238425428774No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTAATAATGTCCTCCTGT4430.13591875556101002No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4400.13499831252109348No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4400.13499831252109348No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4390.13469149817445464No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4380.1343846838278158No Hit
TTATTAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4350.13346424078789923No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4320.1325437977479827No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4310.13223698340134385No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4220.12947565428159422No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4210.12916883993495537No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4200.1288620255883165No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4160.12763476820176112No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4150.12732795385512227No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTCTATATAAGAGAGAAACTACA4140.12702113950848343No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4140.12702113950848343No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4110.12610069646856686No Hit
GTTCAGGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4070.12487343908201148No Hit
GTTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCC4050.12425981038873377No Hit
CAATAGACCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG4030.12364618169545608No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4030.12364618169545608No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4020.12333936734881723No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3990.1224189243089007No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3980.12211210996226185No Hit
TCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTT3950.12119166692234529No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3890.11935078084251219No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3870.1187371521492345No Hit
GTTGTCAATAGACCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3840.11781670910931796No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3770.11566900868284602No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3760.11536219433620717No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3730.11444175129629061No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC3710.11382812260301292No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3670.11260086521645751No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3660.11229405086981867No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3630.11137360782990213No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3630.11137360782990213No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT3630.11137360782990213No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3630.11137360782990213No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3610.11075997913662444No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT3600.1104531647899856No Hit
TCCACAGAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCT3600.1104531647899856No Hit
GCACGGGACCATGCAAGACCTGCACGATACCTGCTCAAGGAACCTCTATG3600.1104531647899856No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA3600.1104531647899856No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC3580.10983953609670788No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC3580.10983953609670788No Hit
ATATAGAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTCTTGA3580.10983953609670788No Hit
TTTACATACTCTGTGGAAGGCGGGTATTCTATATAAGAGAGAAACTACAC3570.10953272175006903No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3570.10953272175006903No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3560.10922590740343019No Hit
CTTCAAGACTAGGCATTATTTACATACTCTGTGGAAGGCGGGTATTCTAT3510.10769183567023594No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3500.10738502132359709No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3490.10707820697695825No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3480.1067713926303194No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3480.1067713926303194No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3460.10615776393704171No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC3460.10615776393704171No Hit
ATATTATCCTGAACAAGCAGTTAATCATTACTTCAAGACTAGGCATTATT3460.10615776393704171No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3450.10585094959040285No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3310.10155554873745896No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3300.10124873439082012No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT3280.10063510569754242No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3270.10032829135090357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)