FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390479
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17470.4473992199324419No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10390.2660834513507769No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10030.2568640054906922No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC8030.20564486182355515No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7630.19540103309012777No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6660.1705597484115663No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6590.1687670783832165No Hit
CTTACAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC6320.16185249398815302No Hit
GCTTAAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA5840.14955989950804013No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCCTTTCCTAAC5580.1429014108313123No Hit
AACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGG5250.1344502521262347No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5220.13368196497122764No Hit
CTTCTAGGGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGT5190.1329136778162206No Hit
CCTTATAAGTTGGCGAGAAAGTAAAAGCCTGCTTAGATTGTATACATGCA5170.13240148637954924No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4970.12727957201283552No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGATACTCCCTTAACTTCAT4970.12727957201283552No Hit
GTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT4910.1257429977028214No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4730.12113327477277908No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4620.11831622187108654No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA4580.1172918389977438No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4450.11396259465937988No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4290.10986506316600893No Hit
GCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT4270.10935287172933755No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4190.10730410598265208No Hit
GTTTTATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4150.10627972310930933No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4120.10551143595430228No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4040.10346267020761679No Hit
TGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCT4000.10243828733427407No Hit
GGGCAAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAGCCAC3910.1001334258692529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)