FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390479
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17470.4473992199324419No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11100.2842662473526105No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9870.2527664739973212No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7540.1930961716251066No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7330.18771816154005722No Hit
CTTACAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC6870.1759377584966157No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6250.1600598239598032No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCCTTTCCTAAC5880.15058428238138286No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5780.148023325198026No Hit
AACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGG5730.14674284660634757No Hit
CCTTATAAGTTGGCGAGAAAGTAAAAGCCTGCTTAGATTGTATACATGCA5600.1434136022679837No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5440.13931607077461272No Hit
GCTTAAGTTTTTGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCA5410.13854778361960565No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5230.13393806068956335No Hit
CTTCTAGGGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGT5160.13214539066121353No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA5100.13060881635119942No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5080.13009662491452806No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4700.12036498761777202No Hit
GTCAAAGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT4680.11985279618110065No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGATACTCCCTTAACTTCAT4650.11908450902609359No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4610.11806012615275085No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4530.11601136040606537No Hit
GCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT4440.11370649894104419No Hit
GTTTTATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4380.11216992463103011No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4040.10346267020761679No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4000.10243828733427407No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4000.10243828733427407No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA3970.10167000017926701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)