FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383960
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13500.3515991249088447No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG11310.2945619335347432No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9610.25028648817585164No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8850.23049275966246485No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7890.20549015522450254No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7790.20288571726221483No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7720.2010626106886134No Hit
GTCTTTGAAGTATGCCTCAAGGTCAGTTGTTGACATTGCTGAGAGTCCAA7440.19377018439420773No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7070.18413376393374312No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6770.17632045004687988No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG6740.17553911865819358No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6640.17293468069590584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6610.17215334930721948No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6500.169288467548703No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6470.16850713616001667No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6440.16772580477133034No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6370.16590269819772893No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6320.16460047921658508No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5950.15496405875612043No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT5640.14689030107302845No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCACAG5590.1455880820918846No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTAAGGTT5500.1432440879258256No Hit
CATGTATACAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5440.14168142514845294No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5260.13699343681633505No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5190.13517033024273362No Hit
GTACAATATGTTCCTGTGGTAAAGTGCCCCAACTTCCAATTACATATCCC5120.1333472236691322No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5000.13022189811438692No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACTGACCT4940.12865923533701426No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG4890.1273570163558704No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4820.125533909782269No Hit
GTTCTATCCCAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAAC4780.12449213459735388No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC4760.12397124700489635No Hit
ATATGTTCCTGTGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAA4670.12162725283883738No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4630.12058547765392227No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT4620.1203250338576935No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA4610.12006459006146472No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4540.11824148348786331No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4510.11746015209917701No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4470.1164183769142619No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAA4440.11563704552557558No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA4410.11485571413688926No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4310.11225127617460152No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4290.11173038858214397No Hit
GTCAACAACTGACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACT4180.10886550682362747No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4120.10730284404625481No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4090.1065215126575685No Hit
GTTGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4060.10574018126888217No Hit
ATCCCAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4050.10547973747265339No Hit
GTGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4030.10495884988019584No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4030.10495884988019584No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4020.10469840608396706No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTA3990.10391707469528076No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3960.10313574330659443No Hit
CAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA3950.10287529951036566No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3880.10105219293676425No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3880.10105219293676425No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3860.1005313053443067No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA3840.10001041775184916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)