FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383960
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13410.3492551307427857No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG12100.31513699343681634No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10440.2719033232628399No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9230.24038962391915825No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8490.22111678299822898No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8370.21799145744348367No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7830.20392749244712993No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7450.1940306281904365No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG6970.18152932597145535No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6920.18022710699031147No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6900.17970621939785394No Hit
GTCTTTGAAGTATGCCTCAAGGTCAGTTGTTGACATTGCTGAGAGTCCAA6750.17579956245442235No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6580.17137201791853318No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6340.1651213668090426No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6270.1632982602354412No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6210.16173559745806854No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6140.15991249088446713No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACTTTACCACAG5960.1552245025523492No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5840.15209917699760392No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5750.14975518283154496No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5680.14793207625794352No Hit
GTACAATATGTTCCTGTGGTAAAGTGCCCCAACTTCCAATTACATATCCC5640.14689030107302845No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACTGACCT5480.14272320033336805No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5320.13855609959370768No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAG5260.13699343681633505No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT5230.13621210542764872No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTAAGGTT5200.1354307740389624No Hit
ATATGTTCCTGTGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAA5110.13308677987290343No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5100.13282633607667466No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5060.13178456089175955No Hit
CATGTATACAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4930.1283987915407855No Hit
GTTCTATCCCAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAAC4900.12761746015209918No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4810.12527346598604022No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4750.12371080320866758No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4750.12371080320866758No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4730.12318991561621002No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4680.12188769663506616No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC4630.12058547765392227No Hit
GTGGTAAAGTGCCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4570.11902281487654964No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA4490.11693926450671946No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4480.11667882071049068No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4390.11433482654443171No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT4310.11225127617460152No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4240.1104281696010001No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4240.1104281696010001No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA4220.10990728200854255No Hit
ATCCCAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4210.10964683821231377No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4180.10886550682362747No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4070.10600062506511095No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA4060.10574018126888217No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4030.10495884988019584No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTAAGTACAATA3960.10313574330659443No Hit
CCTCTATGTTTCCCTCGTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3950.10287529951036566No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA3890.10131263673299302No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAA3880.10105219293676425No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCAGTTGTTGACA3880.10105219293676425No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3870.10079174914053547No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTA3840.10001041775184916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)