FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322583
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16200.502196333966762No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15110.4684065806319614No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA10020.3106177324905528No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9150.2836479293701156No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA8410.26070809683089313No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8290.2569881239866949No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7980.24737819413918277No Hit
GAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCAGCT7810.24210823260990194No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC7390.22908832765520812No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7340.22753833897012554No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7270.2253683548110099No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7070.2191684000706795No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG7010.21730841364858036No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7000.21699841591156385No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6820.2114184566452665No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6650.20614849511598562No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA6340.19653856526847352No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6260.1940585833723414No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6110.18940861731709357No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6070.1881686263690275No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6070.1881686263690275No Hit
TTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGA5990.18568864447289535No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5970.1850686489988623No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC5870.18196867162869712No Hit
CAAATGTTCCACGCATGCGCCGATGGCCTATGGCCAAGCCCCATCCAGTG5850.18134867615466407No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT5840.18103867841764754No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5830.18072868068063103No Hit
CTTTTACACAATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATA5830.18072868068063103No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCGACTTACAAGGCC5820.18041868294361452No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCATGGTGGTCTCCATGC5760.1785586965215154No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5720.1773187055734493No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCCCTCAGGCA5710.1770087078364328No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5660.1754587191513502No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5520.17111875083311892No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT5500.17049875535908587No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5380.16677878251488762No Hit
GCCATGGGAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5370.1664687847778711No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5330.16522879382980504No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA5330.16522879382980504No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5320.16491879609278853No Hit
ATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5310.164608798355772No Hit
CATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAAGGGGCAGCA5280.16367880514472244No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5260.16305880967068942No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA5210.16150882098560682No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5020.15561886398229294No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4910.15220888887511122No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4870.15096889792704513No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA4850.1503489024530121No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT4840.15003890471599557No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4640.14383894997566518No Hit
GTAGAGACCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4610.14290895676461562No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4590.14228896129058258No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4590.14228896129058258No Hit
CAAATGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCATCCAGTG4580.14197896355356607No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4520.14011897713146695No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4440.1376389952353348No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTCGACGA4410.1367090020242852No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGT4270.13236903370605393No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG4270.13236903370605393No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4260.13205903596903742No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTG4260.13205903596903742No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4230.13112904275798787No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4150.1286490608618557No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4140.12833906312483917No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4100.1270990721767731No Hit
GTAAAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC4050.1255490834916905No Hit
GCGCCGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAA4020.12461909028064096No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3990.12368909706959139No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3980.12337909933257488No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3950.12244910612152532No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT3890.1205891196994262No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3860.11965912648837662No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA3830.11872913327732708No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA3810.11810913780329405No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAATTGGATCCTGCGCGGGACATCC3750.11624915138119492No Hit
GTACAACACCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC3740.11593915364417841No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC3730.11562915590716187No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3730.11562915590716187No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA3720.11531915817014535No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3700.11469916269611231No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT3700.11469916269611231No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG3680.11407916722207928No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3630.11252917853699668No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT3590.1112891875889306No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3590.1112891875889306No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3590.1112891875889306No Hit
GGACATCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCG3580.11097918985191407No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3540.109739198903848No Hit
ATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATATT3520.10911920342981496No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3520.10911920342981496No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3490.1081892102187654No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT3470.10756921474473237No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3460.10725921700771583No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCACTTACAGG3440.1066392215336828No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA3440.1066392215336828No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAG3430.10632922379666629No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3420.10601922605964977No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG3420.10601922605964977No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3410.10570922832263326No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3380.10477923511158369No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3360.10415923963755065No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT3360.10415923963755065No Hit
TCACCATACCGCCCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3350.10384924190053413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3300.10229925321545152No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3280.10167925774141849No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3280.10167925774141849No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3280.10167925774141849No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3280.10167925774141849No Hit
GTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG3260.10105926226738546No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3260.10105926226738546No Hit
GACAAAGGATGTCCCGCGCAGGATCCAATTGGCAGCACACCCGAGCAGCC3260.10105926226738546No Hit
ATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT3250.10074926453036892No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCAACCTGGGTGGGAAGTAATTTGG3250.10074926453036892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)