FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322583
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15490.4801864946385891No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14630.4535266892551684No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9960.30875774606845374No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA9390.2910878750585121No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA8940.2771379768927687No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8350.258848110408794No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8340.2585381126717775No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8260.2560581307756453No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG7770.24086824166183587No Hit
GAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCAGCT7710.23900825523973673No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC7690.23838825976570371No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7540.2337382937104559No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7040.21823840685962992No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6950.21544842722648125No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6590.20428850869388654No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCCCTCAGGCA6460.20025853811267177No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC6190.19188859921322574No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6130.19002861279112662No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6100.18909861958007707No Hit
CTTTTACACAATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATA5960.1847586512618458No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5730.17762870331046585No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5640.17483872367731715No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5520.17111875083311892No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5430.16832877119997022No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5430.16832877119997022No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5420.1680187734629537No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA5310.164608798355772No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT5180.16057882777455726No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5130.15902883908947466No Hit
ATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5130.15902883908947466No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5120.15871884135245815No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCATGGTGGTCTCCATGC5070.15716885266737554No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA5060.156858854930359No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5050.1565488571933425No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCGACTTACAAGGCC5040.15623885945632598No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5010.1553088662452764No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4980.15437887303422687No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4940.15313888208616078No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4940.15313888208616078No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4880.15127889566406164No Hit
GCCATGGGAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4810.149108911504946No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4790.14848891603091297No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA4710.14600893413478083No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4700.1456989363977643No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4680.14507894092373125No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4670.14476894318671474No Hit
CATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAAGGGGCAGCA4600.1425989590275991No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4600.1425989590275991No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4570.14166896581654956No Hit
CAAATGTTCCACGCATGCGCCGATGGCCTATGGCCAAGCCCCATCCAGTG4560.14135896807953302No Hit
TTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGA4560.14135896807953302No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG4520.14011897713146695No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4510.13980897939445042No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA4430.13732899749831828No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4290.13298902918008698No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA4290.13298902918008698No Hit
GTAAAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC4180.12957905407290526No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4160.12895905859887224No Hit
GTAGAGACCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4140.12833906312483917No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTCGACGA4140.12833906312483917No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4110.12740906991378964No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4080.12647907670274008No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4030.12492908801765748No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4020.12461909028064096No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC3960.12275910385854184No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3930.12182911064749227No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC3910.12120911517345924No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATACC3850.11934912875136011No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA3850.11934912875136011No Hit
GGACATCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCG3850.11934912875136011No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3820.11841913554031055No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGT3800.11779914006627751No Hit
GTACAACACCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC3780.11717914459224447No Hit
TGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3750.11624915138119492No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3740.11593915364417841No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTG3720.11531915817014535No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC3700.11469916269611231No Hit
CAAATGTTCCACGCATGCGCTGATGGCCTATGGCCAAGCCCCATCCAGTG3670.11376916948506277No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3640.1128391762740132No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3590.1112891875889306No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAATTGGATCCTGCGCGGGACATCC3560.11035919437788104No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3560.11035919437788104No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCACTTACAGG3550.11004919664086453No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT3540.109739198903848No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3500.10849920795578193No Hit
GGTCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCACCCACGGGGCG3490.1081892102187654No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3460.10725921700771583No Hit
GTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG3440.1066392215336828No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3420.10601922605964977No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3420.10601922605964977No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3400.10539923058561673No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT3400.10539923058561673No Hit
ATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATATT3400.10539923058561673No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3400.10539923058561673No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC3390.1050892328486002No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3390.1050892328486002No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3370.10446923737456716No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA3350.10384924190053413No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA3350.10384924190053413No Hit
CTTTACCACAGGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAA3330.1032292464265011No Hit
GCGCCGATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAA3310.10260925095246803No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3310.10260925095246803No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3300.10229925321545152No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3280.10167925774141849No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3270.10136926000440197No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG3270.10136926000440197No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3260.10105926226738546No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT3240.1004392667933524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)