FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648011
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43900.6774576357500104No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15530.23965642558536815No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAA11040.17036747832984317No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAA10630.164040425239695No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10270.15848496398980882No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9180.14166426187209785No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8340.12870151895569673No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT7790.12021400871281505No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7580.11697332298371478No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7450.11496718419903365No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA7350.11342400051850973No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC7090.10941172294914747No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6630.10231307801873733No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA6610.10200444128263256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)