FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451698
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT18150.40181714331256724No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16290.36063918813012236No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG15040.3329658311526728No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG12980.287360138853836No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12810.2835965623049028No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12640.2798329857559697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11670.2583584607414689No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11050.2446324756806539No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10910.24153305969917951No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10880.24086889913172074No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10580.23422729345713286No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC10390.23002094319656052No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10090.22337933752197264No Hit
CATTTAAACCCTAATAAAACCAAACGATGGGGTTACTCCCTTCACTTCAT9810.21718050555902396No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCTTG9220.20411868106566777No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9040.20013371766091503No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT8920.19747707539107986No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8700.19260656456304875No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8690.19238517770722915No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA8640.19127824342813118No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8540.18906437486993524No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT8540.18906437486993524No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8510.18840021430247644No Hit
AAGTTAAGGGAGTAACCCCATCGTTTGGTTTTATTAGGGTTTAAATGTAT8420.18640773260010007No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8270.18308692976280613No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8120.17976612692551217No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8100.17932335321387297No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8090.1791019663580534No Hit
CCACAATTCTTTGACACACTTTCCAATCAATAGGTCTATTTACAGGCAGT8010.17733087151149662No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7970.17644532408821823No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG7870.17423145553002228No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7860.17401006867420268No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7820.17312452125092428No Hit
CCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7810.17290313439510469No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7440.16471182072977963No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7340.16249795217158367No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7300.1616124047483053No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA7230.1600626967575681No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC7120.15762744134355255No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA7090.15696328077609376No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6800.15054306195732547No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATAAAC6670.1476650328316707No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6670.1476650328316707No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6610.14633671169675316No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6590.14589393798511396No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6580.14567255112929436No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT6540.14478700370601597No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6470.1432372957152788No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATA6330.14013787973380445No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6320.13991649287798485No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6300.13947371916634566No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA6300.13947371916634566No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6260.13858817174306726No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAAT6230.13792401117560849No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6130.13571014261741252No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA5980.13238933978011858No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC5910.1308396317893814No Hit
TGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5880.1301754712219226No Hit
CCCTCATAAAACCAAACGATGGGGTTACTCCCTTCACTTCATGGGATATG5830.1290685369428246No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5710.1264118946729895No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5550.12286970497987593No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5490.12154138384495837No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5470.12109861013331917No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG5430.12021306271004079No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA5410.11977028899840159No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC5390.11932751528676239No Hit
CCTTTACCCCGTTGCCCGGCAACGATCAGGTCTCTGCCAAGTGTTTGCTG5340.11822058100766442No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5340.11822058100766442No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5340.11822058100766442No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT5330.1179991941518448No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5280.11689225987274685No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5260.11644948616110765No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5250.11622809930528805No Hit
CTCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAG5210.11534255188200968No Hit
GGGTTACTCCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACTT5190.11489977817037048No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5130.11357145703545289No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5120.1133500701796333No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5100.1129072964679941No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT5060.11202174904471572No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4990.11047204105397854No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4960.10980788048651974No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4930.10914371991906098No Hit
CCCACAATTCTTTGACACACTTTCCAATCAATAGGTCTATTTACAGGCAG4910.10870094620742178No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4910.10870094620742178No Hit
GATTAACTGCATGTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGC4860.10759401192832378No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4770.10560153022594741No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4730.10471598280266904No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4720.10449459594684946No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4720.10449459594684946No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4710.10427320909102984No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCC4630.10250211424447309No Hit
TTGACACACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4610.10205934053283389No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4540.10050963254209672No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC4530.10028824568627712No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4530.10028824568627712No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4530.10028824568627712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)