FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606620
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA28510.46998120734562No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC22460.3702482608552306No Hit
CAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGG19820.32672842965942434No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCGCCAATCGGCAGT18560.3059576011341532No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCGTG17080.2815601200092315No Hit
GGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCCGTGCTGGTTG16830.27743892387326496No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16720.27562559757343974No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG16650.2744716626553691No Hit
ATATTGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAG15990.26359170485641753No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG15900.26210807424746957No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACAGCCAATTACATATCCCATGAA15820.2607892914839603No Hit
ATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAGTTACTG15590.2569977910388711No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14770.243480267712901No Hit
GGTGTTTCGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCCGTGCTGGTTG14060.23177607068675612No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT13630.22468761333289375No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13570.2236985262602618No Hit
CCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA12970.21380765553394215No Hit
ATATTAACGTTGACATAGTTGACTATTAATTCCCTGGATGCTGGATCTTC12640.2083676766344664No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT12120.19979558867165603No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATACAGTGGAA11940.19682832745376017No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11720.19320167485410966No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC11610.19138834855428438No Hit
ATATGGAGCAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA11450.18875078302726583No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTG11430.18842108733638852No Hit
GAATACAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC11420.18825623949094986No Hit
GGTCTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT11230.1851241304276153No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC11130.1834756519732287No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA11050.1821568692097194No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT11030.1818271735188421No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG10970.18083808644621016No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10780.1777059773828756No Hit
CAAATGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCCAT10760.1773762816919983No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT10730.1768817381556823No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG10380.17111206356532918No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA10170.16765025881111734No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATGAGAGGACAAACGGGCAAC10150.16732056312024002No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9880.1628696712933962No Hit
CGAAACACCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGG9790.16138604068444826No Hit
ACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAGATCT9780.1612211928390096No Hit
GGAATGAACCCTGGTCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG9770.16105634499357094No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9550.15742969239392043No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9530.1570999967030431No Hit
CCTTTGGAGTGTGGATTCGCACTCCTGCTCCATATAGACCACCAAATGCC9520.15693514885760443No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG9460.15594606178497247No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG9430.1554515182486565No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC9420.15528667040321784No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9390.15479212686690186No Hit
GCTGTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT9310.15347334410339256No Hit
CCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA9200.1516600178035673No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTGCGGGTCACCATATT9060.14935214796742607No Hit
CTCCAAAGGACACCAAATATTCAAGGACAGTTTCTCTCCCAAAAGTAATA8790.14490125614058225No Hit
ATGTATACTTGCGGGAAAGCACGACAGAATTGTCAGTCCCGATGAGTTTT8780.1447364082951436No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8650.142593386304441No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8440.13913158155022914No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA8400.13847219016847448No Hit
AAGTAATACAGGAAAGGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC8370.13797764663215853No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8330.13731825525040386No Hit
GTGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG8270.1363291681777719No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8260.13616432033233325No Hit
ACAATATCCTCCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCAATT8230.13566977679601727No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGA8190.13501038541426263No Hit
CCTGCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8080.13319705911443738No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGTCT8070.13303221126899872No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7810.12874616728759355No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7760.12792192806040026No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7750.12775708021496157No Hit
GTATAAAGAATTTGGAGCTTCTATGGAGTTACTCTCTTTTTTGCCTTCTG7690.12676799314232962No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7670.1264382974514523No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7530.12413042761531107No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7420.12231710131548582No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG7300.12033892717022189No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTTC7270.1198443836339059No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATACAGTGGAACACCCCCACTTT7250.11951468794302858No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7250.11951468794302858No Hit
CGTCCTTTGTTTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCCTCC7210.11885529656127393No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT7190.11852560087039661No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7160.11803105733408065No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7150.11786620948864199No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAACTT7090.11687712241601003No Hit
GCCAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTT7060.11638257887969404No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA7010.11555833965250074No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7000.11539349180706207No Hit
ATATGGCCGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAG6900.11374501335267549No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6870.1132504698163595No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC6860.11308562197092084No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG6840.11275592628004352No Hit
GGATCGGCAGAGGAGACACAAAGGTTCCACGCACGCGCTGATGGCCGATG6810.11226138274372753No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAA6790.11193168705285021No Hit
GTATTACTTTTGGGAGAGAAACTGTCCTTGAATATTTGGTGTCCTTTGGA6760.11143714351653425No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACCTTTCCT6750.11127229567109558No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCCATCAGGCA6740.11110744782565692No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT6570.1083050344531997No Hit
CTCCTGCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6560.10814018660776104No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG6520.10748079522600638No Hit
GAATTAATAGTCAACTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6520.10748079522600638No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6470.1066565559988131No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6450.10632686030793577No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG6440.10616201246249712No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6440.10616201246249712No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6410.10566746892618113No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6400.10550262108074249No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG6380.10517292538986517No Hit
GTAATTGGCTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT6380.10517292538986517No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6320.1041838383172332No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACATAGCGCC6310.10401899047179453No Hit
GGTTGGGGCTTGGCCATCGGCCATCAGCGCGTGCGTGGAACCTTTGTGTC6290.10368929478091721No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG6210.10237051201740795No Hit
GGTCTATATGGAGCAGGAGTGCGAATCCACACTCCAAAGGACACCAAATA6210.10237051201740795No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6190.10204081632653061No Hit
GCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAGT6150.10138142494477598No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6100.10055718571758267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)