FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498527
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24320.4878371682978054No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG21420.4296657954333466No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21060.42244452156051726No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTCGT19910.3993765633556457No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC18140.3638719668142347No Hit
CCTTTTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAC18020.3614648755232916No Hit
CCTCTATATTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC13640.2736060434038678No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGTATTAGAGGACAAACGGGCAAC12760.25595404060361826No Hit
ATATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG12640.25354694931267513No Hit
CTTTAGGACATGGACATCGACACGTATAAAGAATTTGGAGCTTCTGTGGA12370.24813099390805313No Hit
GTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA12050.2417120837988715No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGCTCAGATCACCATACAG11460.2298772182850678No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCACCTCTATA11360.22787130887594853No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10730.2152340795984972No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10710.2148328977166733No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAA10620.213027579248466No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTACGGGTCACCATATT10600.2126263973666421No Hit
TCCAAAGTGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10300.2066086691392843No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA10210.204803350671077No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG10130.2031986231437816No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT9900.19858503150280726No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9830.1971808949164238No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTCCATGC9750.1955761673891284No Hit
GTTGATAGATGTACTCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG9370.18795371163447516No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9190.1843430746980605No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9170.18394189281623663No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9040.1813342105843816No Hit
GAAATGATGTATACTTGCGGGAGAGGACGACAGAATTGTCAGTCCCGATG8790.17631943706158343No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8720.17491530047519993No Hit
ATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATCTA8650.17351116388881646No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG8290.1662898900159871No Hit
ATGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTA8280.1660892990750752No Hit
CTATATTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCACC8160.16368220778413203No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8130.1630804349613963No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATACCAGGATCATCAACAACC8110.16267925307957243No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGGACGACAGAAT8080.16207748025683663No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7910.1586674342613339No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTCCATATAGACCTCCAAATGCC7570.1518473422703284No Hit
GTTGCAAGTATGCCTTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCGTG7440.14923966003847336No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7320.14683256874753023No Hit
CTGTAAGGGGCCCCACAAATTGAGTACATCTATCAACAATGTCCTCCTGC7280.14603020498388253No Hit
CCCCAACAGTCCCCCGTGGGGAGGAATGAATCCCGGCCCGAATGCTCCCA7280.14603020498388253No Hit
GATGTATACTTGCGGGAGAGGACGACAGAATTGTCAGTCCCGATGAGTTT7230.14502725027932287No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7170.14382370463385133No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC7160.1436231136929394No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7150.14342252275202746No Hit
ATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTCGTCTGGA7130.14302134087020363No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7120.1428207499292917No Hit
CCTTGTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAC7110.14262015898837976No Hit
ATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAATTACTG7090.14221897710655593No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7050.14141661334290823No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7050.14141661334290823No Hit
GTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTT7030.14101543146108436No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA7010.1406142495792605No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6980.14001247675652473No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTC6970.1398118858156128No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6950.13941070393378893No Hit
CATCATTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGG6880.13800656734740546No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6870.13780597640649353No Hit
CCTCCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAAT6850.1374047945246697No Hit
GAAAAGATGTATACTTGCGGGAGAGGACGACAGAATTGTCAGTCCCGATG6850.1374047945246697No Hit
CTCAAGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGAT6820.1368030217019339No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6750.13539888511555043No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAACTGGCAAACTCCTT6720.13479711229281463No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6660.1335935666473431No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6480.1299829297109284No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6450.1293811568881926No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCT6410.1285787931245449No Hit
AGTCTGTACAACACCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTT6360.1275758384199853No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6360.1275758384199853No Hit
GTACAACAGGCGGAGAAGCGGCCGGTAGAGCCCCAAGCGGCCCCGGGAGG6300.1263722927745137No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6290.1261717018336018No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA6240.12516874712904216No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6180.1239652014835706No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTCCATATACTGTTTACTT6160.12356401960174676No Hit
GTATATGGACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG6150.12336342866083481No Hit
GCGTCGCAGAAGATCTCAATCTCGGGGATCTCAATGTTAGTATTCCTTGG6080.12195929207445134No Hit
GTTTACAGGGAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6070.1217587011335394No Hit
GTACAACACCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTTTTGTC6070.1217587011335394No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6050.12135751925171556No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAAAAGGAGTTT6040.12115692831080364No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5980.11995338266533206No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG5950.11935160984259628No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5920.1187498370198605No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG5860.11754629137438895No Hit
GTATGGATCGGCAGCGGAGACACAAAGGTTCCACGCACGCGCTGATGGCC5760.11554038196526968No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5740.11513920008344583No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5680.11393565443797427No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCATTTAC5670.11373506349706235No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5660.11353447255615043No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC5640.11313329067432656No Hit
GGGTAAAGGTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5600.11233092691067886No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5540.11112738126520731No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA5460.10952265373791188No Hit
CCATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATC5460.10952265373791188No Hit
GAAAACAAGGAGTTTGCCAGTTAGGATTGAAGCAAGGTACCGTAGAAGAA5360.10751674432879263No Hit
GTATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5350.1073161533878807No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5320.10671438056514491No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5280.10591201680149721No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5260.10551083491967335No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5220.10470847115602565No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5210.10450788021511373No Hit
TCATTAACCTGGTCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG5200.1043072892742018No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5140.10310374362873023No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5110.10250197080599445No Hit
ACACATAGCGCCTCATTTTACGGGTCACCATATTCTTGGGAACAAGATCT5100.10230137986508253No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATACCAGGATC5080.1019001979832587No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5050.1012984251605229No Hit
GAATATGGTGACCCGTAAAATGAGGCGCTATGTGTGGACTCTCTTTTATA5040.10109783421961098No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5010.1004960613968752No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTT5010.1004960613968752No Hit
CACTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACATAGCGCC5000.10029547045596328No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG5000.10029547045596328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)