FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498527
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20800.4172291570968072No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG20760.4164267933331595No Hit
GTATATGGACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAG19700.39516415359649526No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTCGT18900.37911687832354113No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16760.33619041696838886No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16560.3321785981501504No Hit
CCTTTTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAC16420.3293703249773834No Hit
CTTTAGGACATGGACATCGACACGTATAAAGAATTTGGAGCTTCTGTGGA15290.3067035486543357No Hit
CCTCTATATTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC13790.2766149075175467No Hit
GTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11880.23830203780336873No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGTATTAGAGGACAAACGGGCAAC11660.23388903710330633No Hit
ATATAGAGGTGCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG11640.2334878552214825No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGCTCAGATCACCATACAG11610.2328860823987467No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG11220.22506303570318156No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCACCTCTATA10610.21282698830755406No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10590.21242580642573022No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAA10440.2094169423120513No Hit
TCCAAAGTGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10300.2066086691392843No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT10150.20359980502560543No Hit
ATATAAAAGAGAGTCCACACATAGCGCCTCATTTTACGGGTCACCATATT9920.19898621338463116No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG9870.19798325868007147No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9680.1941720308027449No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA9600.1925673032754495No Hit
GTTGATAGATGTACTCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG9550.19156434857088983No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9290.18634898410717976No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8760.17571766423884763No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8750.17551707329793573No Hit
ATGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTA8270.16588870813416323No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8120.16287984402048433No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7970.15987097990680546No Hit
CTGTAAGGGGCCCCACAAATTGAGTACATCTATCAACAATGTCCTCCTGC7810.15666152485221463No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT7780.15605975202947886No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7740.15525738826583116No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7690.1542544335612715No Hit
CTATATTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCACC7570.1518473422703284No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7460.1496408419202972No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTCCATGC7300.14643138686570636No Hit
GTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTT7240.14522784122023483No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7220.14482665933841096No Hit
AGTCTGTACAACACCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTT7220.14482665933841096No Hit
ATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTCGTCTGGA7170.14382370463385133No Hit
ATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAATTACTG7140.14322193181111553No Hit
GTTGCAAGTATGCCTTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCGTG7070.14181779522473206No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCT7060.14161720428382013No Hit
ATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATCTA7030.14101543146108436No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6980.14001247675652473No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGTGTGTGTAAATAATGTCTC6960.13961129487470086No Hit
GTACAACACCTTGAGTCCATTTATGCCGCTGTTACCAATTTTCTTTTGTC6930.1390095220519651No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6890.1382071582883174No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATACCAGGATCATCAACAACC6840.13720420358375773No Hit
CCTCCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAAT6810.13660243076102196No Hit
CCCCAACAGTCCCCCGTGGGGAGGAATGAATCCCGGCCCGAATGCTCCCA6640.13319238476551923No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6640.13319238476551923No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTCCATATAGACCTCCAAATGCC6590.1321894300609596No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6510.1305847025336642No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6480.1299829297109284No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCATTTAC6460.12958174782910453No Hit
CATCATTTCCATGGCTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGG6380.12797702030180913No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAACTGGCAAACTCCTT6380.12797702030180913No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG6360.1275758384199853No Hit
ATATGGACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG6320.12677347465633756No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6240.12516874712904216No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6200.12436638336539446No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA6180.1239652014835706No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC6150.12336342866083481No Hit
CCTTGTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAC6150.12336342866083481No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6150.12336342866083481No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6060.12155811019262748No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6030.1209563373698917No Hit
CTGCCACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCT6030.1209563373698917No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6020.12075574642897978No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5980.11995338266533206No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5970.11975279172442015No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATGGACATCGA5960.1195522007835082No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5960.1195522007835082No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5950.11935160984259628No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTCCATATACTGTTTACTT5950.11935160984259628No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5890.11814806419712473No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5890.11814806419712473No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5880.1179474732562128No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5870.11774688231530088No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATA5840.11714510949256508No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5830.11694451855165316No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5790.11614215478800546No Hit
CTCAAGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGAT5790.11614215478800546No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5770.11574097290618163No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5660.11353447255615043No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAAAAGGAGTTT5600.11233092691067886No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5600.11233092691067886No Hit
GCTTTAGGACATGGACATCGACACGTATAAAGAATTTGGAGCTTCTGTGG5590.11213033596976693No Hit
GTTTACAGGGAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5580.11192974502885501No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5490.11012442656064766No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5410.10851969903335225No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA5380.10791792621061647No Hit
GGGTAAAGGTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5360.10751674432879263No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG5350.1073161533878807No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5320.10671438056514491No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG5310.106513789624233No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5200.1043072892742018No Hit
GTATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5130.10290315268781831No Hit
GAAAACAAGGAGTTTGCCAGTTAGGATTGAAGCAAGGTACCGTAGAAGAA5090.10210078892417061No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5070.10169960704234675No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5000.10029547045596328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)