FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428329
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACATAAATC18010.42047117986407645No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT16940.3954903823929736No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG16820.39268879762985925No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTGTCAACACTTCCGGA15470.361170969044823No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGAACTTGCTGAAAGTCCAA15320.35766898809093006No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCGTG12510.2920652115546694No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAACCACATTGTGTGAAA11690.2729210490067215No Hit
GTAGTAGGCTATGTCAACGTTAATATGGGCATAAAACTCAGACAACTATT11300.26381589852659987No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATGCGTTGACATACT10750.2509753016956592No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10340.24140322042168522No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9780.22832915819381833No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9750.22762876200303975No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATGAGAGGACAAACGGGCAAC9730.22716183120918731No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9620.2245937118429992No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8990.20988539183664892No Hit
GTATTATCCAGAGCATGTGGTTAATCATTACTTCCAGACGAGACATTATT8970.20941846104279654No Hit
GCACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8930.2084845994550918No Hit
AGTCTAGACTCTGTGGTATTGTGAGGGTTTTTGTCAACAAGAAAAACCCC8820.20591648008890362No Hit
GTAAAAGCCTGTTTGGCTTGTATACATGCATATAAAGGCATCAAAGCAGG8780.20498261850119887No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8740.20404875691349406No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC8550.19961291437189635No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAA7930.1851380597624723No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACCACA7810.18233647499935796No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT7670.17906795944239126No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7540.1760329092823507No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGTC7530.1757994438854245No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAACATTCG7320.17089667054997443No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7030.16412617403911478No Hit
GGAAAATACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT6990.16319231245141003No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6930.16179152006985284No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6920.16155805467292667No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC6780.15828953911595992No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGG6560.15315330038358366No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA6520.15221943879587888No Hit
TTCTAAGACAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCT6510.15198597339895267No Hit
ATCCAGAGCATGTGGTTAATCATTACTTCCAGACGAGACATTATTTACAC6470.1510521118112479No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6450.15058518101739551No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6390.14918438863583836No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6220.14521547688809303No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6080.1419469613311263No Hit
CCTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGAACTT6040.14101309974342152No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6040.14101309974342152No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6020.14054616894956914No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAGAAGGAGTTT5890.13751111878952862No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5850.13657725720182382No Hit
GGATAACCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5840.13634379180489764No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTCATTCCAGGATCATCAACAACC5820.13587686101104524No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5730.1337756724387095No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5680.1326083454540785No Hit
CTCCTACTCCCACCTTGTTGGCGTCCGGCCAGTTGTCCTTGTGCGGGTTG5670.13237488005715234No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5660.13214141466022614No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5510.1286394337063332No Hit
GGCCTTTACTTTCTCGTCAACTTACAAGGCCTTTCTAAGACAACAATATC5490.12817250291248083No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5480.12793903751555463No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5460.12747210672170226No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5410.12630477973707127No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCCGTG5350.1249039873555141No Hit
GCCTGAGGATGCGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5310.12397012576780932No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5280.12326972957703074No Hit
ATCCAGCATCCAGGGAAGCAGTAGTAGGCTATGTCAACGTTAATATGGGC5220.12186893719547356No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5160.1204681448139164No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5070.11836695624158064No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5070.11836695624158064No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGGCAACTTACAAGGCC5070.11836695624158064No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5070.11836695624158064No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4910.11463150989076154No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC4910.11463150989076154No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4900.11439804449383535No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4860.11346418290613057No Hit
CCCCAACAGTCCCCCGTGGGGAGGGGTGAACCCTGGCCCGAATGCTCCTA4820.11253032131842577No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4800.11206339052457341No Hit
GAGTAGGAGCATTCGGGCCAGGGTTCACCCCTCCCCACGGGGGACTGTTG4790.1118299251276472No Hit
ATTTAGTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC4760.11112952893686862No Hit
GTGGGGGAAAGTCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4720.11019566734916383No Hit
GTCTTCGATAAGATTGACGATATGGCTGAGACAGTATTCTGAGCAGGGCT4710.10996220195223766No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4710.10996220195223766No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4700.10972873655531147No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4680.10926180576145907No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4640.1083279441737543No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTGTCTT4600.1073940825860495No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4600.1073940825860495No Hit
GCCTACTACTGCTTCCCTGGATGCTGGATCGTCCAAATTACTTCCCACCC4590.10716061718912331No Hit
CATATAAAGGCATCAAAGCAGGATAACCACATTGTGTGAAAGGGGCGGCA4590.10716061718912331No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4560.10646022099834473No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGC4530.10575982480756614No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGTCCTA4530.10575982480756614No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTGTCAACACTTCCGGAAAC4500.10505942861678756No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCCGAAAATCCAGATTGGGACCT4460.1041255670290828No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA4370.10202437845674703No Hit
GTTCAATGGTTCGTAGGACTTTCCCCCACTGTCTGGCTTTCAGTTATATG4370.10202437845674703No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4360.10179091305982085No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT4340.10132398226596845No Hit
ATCCTGGAATGAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGA4290.10015665528133746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)