FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284951
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC28420.9973644591526263No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG20990.7366178746521332No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17750.6229141150583785No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13430.47130910226670547No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11270.39550659587086906No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10370.3639222182059372No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9550.33514534077788816No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8600.30180627546490446No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7800.27373127309607614No Hit
TTTGCCAGATCCAGCATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTA7310.2565353341451688No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6930.24319970801997534No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6920.24284877049036502No Hit
CTCTTTGGAAGGCGGGTATTTTATATAAAAGAGAGTCCACACGTAGCGCC6840.24004127025348218No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTACGCCTCACCATACGG6790.2382865826054304No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6670.23407533225010616No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6510.2284603317763405No Hit
TATCCACATTGTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAATTCG6490.2277584567171198No Hit
GTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6380.2238981438914059No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5930.20810595505893997No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5800.20354376717400535No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGTGTGAAA5610.19687595411140862No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC5600.19652501658179827No Hit
ATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAACTCACCCC5570.19547220399296722No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5380.1888043909303705No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5360.1881025158711498No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5330.1870497032823187No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAA5330.1870497032823187No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT5320.18669876575270836No Hit
CTTGATTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTAT5190.18213657786777376No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG5170.18143470280855303No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC5020.17617063986439774No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5010.1758197023347874No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4950.17371407715712525No Hit
ATGTAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG4820.16915188927219066No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4810.1688009517425803No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4790.1680990766833596No Hit
GGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATA4750.16669532656491817No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGG4730.16599345150569747No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4700.16494063891686642No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4680.16423876385764571No Hit
GTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGGATGTAGAGGA4650.16318595126881463No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA4590.1610803260911525No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4530.1589747009134904No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4450.15616720067660755No Hit
CCTTTCACACAATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTAT4410.15476345055816615No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4410.15476345055816615No Hit
ATTGTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4190.14704282490673834No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4180.146691887377128No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4140.14528813725868658No Hit
GAATACTGTCTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4090.14353344961063483No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCAGGCGCTGATGGCC4090.14353344961063483No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTACCATT4030.1414278244329727No Hit
GCTCAGAATACTGTCTCTCCCATATCGTCAATCTTATCGAAGACTGGGGA4010.14072594937375196No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT3950.13862032419608986No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC3900.13686563654803807No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3890.13651469901842772No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCAGTG3870.13581282395920702No Hit
ATTTAGTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC3860.13546188642959667No Hit
CCTCTACATCCTGCTGCTATGCCTCATCTGCTTGTTGGTTCTTCTGGACT3810.1337071987815449No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATATGTT3800.13335626125193456No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3800.13335626125193456No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA3780.13265438619271383No Hit
GTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAG3770.13230344866310348No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGGAAACTTCCTGTAA3740.13125063607427243No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3670.12879407336699994No Hit
TCACCATACGGCTCTCAGGCAAGCTATTGTATGTTGGGGTGAGTTGATGA3550.12458282301167568No Hit
GCCTTAGAGTCTCCAGAACATTGTACGCCTCACCATACGGCTCTCAGGCA3540.12423188548206533No Hit
GACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTGTGTCT3530.12388094795245498No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3480.12212626030440321No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA3450.12107344771557216No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC3450.12107344771557216No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3440.12072251018596179No Hit
CCACAAGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAA3410.11966969759713074No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3400.11931876006752039No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3400.11931876006752039No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3400.11931876006752039No Hit
GTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3390.11896782253791004No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC3370.11826594747868932No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTTAGATCTTCTGCGAC3360.11791500994907897No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTCCCATATCGTCA3350.11756407241946862No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT3320.11651125983063754No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3310.11616032230102719No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3280.11510750971219615No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA3280.11510750971219615No Hit
GCCTGAGGATGCGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTTCCT3270.11475657218258577No Hit
GCGTTCTATCATATTCCTCTACATCCTGCTGCTATGCCTCATCTGCTTGT3260.11440563465297542No Hit
GGATATCCACATTGTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAAT3190.11194907194570294No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3180.11159813441609259No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3100.10879063417920977No Hit
CTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTGCCGAACATG3080.10808875911998905No Hit
GTTCAAGCCTCCGAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA3070.1077378215903787No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC3070.1077378215903787No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3040.10668500900154762No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGC3040.10668500900154762No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3040.10668500900154762No Hit
GGTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG3030.10633407147193727No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3020.10598313394232692No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3020.10598313394232692No Hit
GTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAG2990.10493032135349588No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGCAGATGAGAAGGCACAG2910.10212282111661303No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC2900.10177188358700268No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG2890.10142094605739232No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGCACAGGGTCCCC2890.10142094605739232No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT2880.10107000852778197No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2870.10071907099817162No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCGAGCTGTGC2860.10036813346856126No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTCCCATATCGTCA2850.1000171959389509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)