FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284951
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC23550.8264578822323837No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG20980.7362669371225228No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15940.5593944221989043No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13080.45902628873034307No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10690.3751522191534685No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG10620.372695656446196No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9730.3414622163108745No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9340.3277756526560707No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8690.3049647132313977No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7100.24916564602335137No Hit
TTTGCCAGATCCAGCATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTA6890.24179595790153394No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6670.23407533225010616No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTACGCCTCACCATACGG6340.22249439377296448No Hit
CTCTTTGGAAGGCGGGTATTTTATATAAAAGAGAGTCCACACGTAGCGCC6330.2221434562433541No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5930.20810595505893997No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC5920.2077550175293296No Hit
GTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5860.2056493923516675No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG5800.20354376717400535No Hit
TATCCACATTGTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAATTCG5780.20284189211478465No Hit
CCTCTACATCCTGCTGCTATGCCTCATCTGCTTGTTGGTTCTTCTGGACT5760.20214001705556395No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAA5570.19547220399296722No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5510.19336657881530508No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5500.19301564128569473No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5470.19196282869686365No Hit
CTTGATTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTAT5290.1856459531638773No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5170.18143470280855303No Hit
ATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAACTCACCCC5150.18073282774933233No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5050.1772234524532288No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5030.1765215773940081No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGTGTGAAA5020.17617063986439774No Hit
GCTCAGAATACTGTCTCTCCCATATCGTCAATCTTATCGAAGACTGGGGA5000.17546876480517704No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA4960.17406501468673563No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4780.16774813915374925No Hit
GAATACTGTCTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4560.16002751350232144No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC4550.15967657597271112No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT4540.15932563844310074No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4520.15862376338388004No Hit
GGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATA4510.1582728258542697No Hit
CCTTTCACACAATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTAT4390.15406157549894545No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4340.15230688785089366No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4330.1519559503212833No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4260.14949938761401083No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTACCATT4240.14879751255479012No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4150.14563907478829693No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCAGGCGCTGATGGCC4120.14458626219946588No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4090.14353344961063483No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4030.1414278244329727No Hit
ATTGTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4010.14072594937375196No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC3980.1396731367849209No Hit
GCGTTCTATCATATTCCTCTACATCCTGCTGCTATGCCTCATCTGCTTGT3920.1375675116072588No Hit
ATGTAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG3850.13511094889998632No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3820.13405813631115526No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3760.13195251113349313No Hit
GCCTTAGAGTCTCCAGAACATTGTACGCCTCACCATACGGCTCTCAGGCA3720.1305487610150517No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA3700.129846885955831No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTTAGATCTTCTGCGAC3680.1291450108966103No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTCCCATATCGTCA3640.12774126077816889No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATATGTT3630.12739032324855853No Hit
GTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3620.12703938571894818No Hit
GACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTGTGTCT3620.12703938571894818No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3550.12458282301167568No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGG3530.12388094795245498No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3510.12317907289323428No Hit
CCACAAGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAA3510.12317907289323428No Hit
GTCCAGAAGAACCAACAAGCAGATGAGGCATAGCAGCAGGATGTAGAGGA3460.12142438524518251No Hit
ATTTAGTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC3460.12142438524518251No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3450.12107344771557216No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA3430.12037157265635144No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT3430.12037157265635144No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3410.11966969759713074No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC3370.11826594747868932No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC3370.11826594747868932No Hit
CTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTGCCGAACATG3340.11721313488985827No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3330.11686219736024789No Hit
GTTCAAGCCTCCGAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA3320.11651125983063754No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGC3310.11616032230102719No Hit
GCCTGAGGATGCGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTTCCT3290.11545844724180648No Hit
GTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAG3290.11545844724180648No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3240.11370375959375471No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3230.11335282206414436No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3230.11335282206414436No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3220.11300188453453401No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3200.1123000094753133No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGGAAACTTCCTGTAA3160.11089625935687189No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCGAGCTGTGC3150.11054532182726153No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA3130.10984344676804082No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3080.10808875911998905No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT3060.10738688406076835No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3050.107035946531158No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3050.107035946531158No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3030.10633407147193727No Hit
TCACCATACGGCTCTCAGGCAAGCTATTGTATGTTGGGGTGAGTTGATGA3030.10633407147193727No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3000.10528125888310622No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2990.10493032135349588No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT2980.1045793838238855No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG2970.10422844629427515No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG2940.1031756337054441No Hit
GGATATCCACATTGTGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAAT2940.1031756337054441No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC2890.10142094605739232No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCTT2890.10142094605739232No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGCTGGATCTGG2850.1000171959389509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)