FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447715
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23160.5172933674324068No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17520.3913203712182974No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG16880.37702556313726365No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT16150.36072054766983463No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14640.3269937348536457No Hit
GTTGTGAGTATGCCCTGCGCCTGAGGGCTCCACCCCAACAGGCCCCCATG14440.3225266073283227No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13500.30153110795930443No Hit
GAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCT11460.25596640720100955No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCACACAAGGA11420.25507298169594494No Hit
ATGTAAGACCTTGGGCAAGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC10610.2369811152183867No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA10520.23497090783199132No Hit
CTCACCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTT10520.23497090783199132No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9850.22000603062215918No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT9780.21844253598829613No Hit
GGATAATAGGGTTTGATTCCTTTATCCAAGGGCAAGTATTTGGTAACTTT9680.21620897222563462No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9640.21531554672057002No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGACAGCACGACAGAAT9490.21196520107657774No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9230.2061579352936578No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA9220.20593457891739164No Hit
CTATTATCCTGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT9030.20169080776833476No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTACCGATTACATAACCCATGAA8390.18739599968730106No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACACACT8010.17890845738918731No Hit
GGCTCTGAATGCAGGGTCCAATTGATGATCGGGGAAGAATCCCAGTGGAT7870.1757814681214612No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCAATCAGGCA7800.17421797348759815No Hit
GTAAACACACAGTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGC7730.17265447885373508No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7710.17220776610120278No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7510.16774063857587976No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7420.16573043118948438No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC7410.16550707481321822No Hit
GTTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG7310.1632735110505567No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7260.16215672916922594No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA7200.16081659091162903No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCGCGCATGCGCTGATGGCC7190.1605932345353629No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7130.159253096277766No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7120.15902973990149985No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT7070.15791295802016908No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7010.15657281976257217No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA6830.15255240498978145No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6830.15255240498978145No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6800.151882335860983No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6750.15076555397965222No Hit
TTGTACACCTCACCATACGGCAATCAGGCAAGCTATTCTGTGTTGGGGTG6720.15009548485085378No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6670.14897870296952304No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6660.14875534659325687No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6650.14853199021699073No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6640.14830863384072457No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAAC6470.14451157544419999No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6380.14250136805780464No Hit
GTATTTGGTAACTTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6250.13959773516634466No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG6240.1393743787900785No Hit
ATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGTTTT6200.13848095328501392No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG6190.13825759690874775No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6180.1380342405324816No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6100.13624738952235238No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5940.13267368750209396No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5930.13245033112582782No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5920.13222697474956166No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5920.13222697474956166No Hit
GTCCAAGGAATACTAACATTTAGATTCCCGAGAGTGAGATCTTCTGCGAC5920.13222697474956166No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5910.13200361837329552No Hit
GCTCAGAATACTGTCTCAGCCATATCATCAACCTTCTCGAAGACTGGGGA5890.1315569056207632No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5880.13133354924449706No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5860.13088683649196475No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5810.129770054610634No Hit
CTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTT5800.12954669823436785No Hit
GTTGACGATATGGCTGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGA5770.12887662910556938No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5570.12440950158024637No Hit
CCCCAGAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTGT5560.12418614520398022No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT5530.12351607607518175No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5480.122399294193851No Hit
CCTTATGAGTCCAAGGAATACTAACATTTAGATTCCCGAGAGTGAGATCT5480.122399294193851No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG5450.12172922506505256No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5360.11971901767865718No Hit
CCATATTCCTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5330.11904894854985872No Hit
ACATAATTTGATTTTGTGTACAATATGTTCCTGTGGCAAGGAACCCCAAC5310.11860223579732643No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5280.11793216666852797No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5270.11770881029226181No Hit
GGTCTATGCCAAGTGTTTGCGGACGCAACCCCCACTGGTTGGGGCTTGGC5270.11770881029226181No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCAGG5260.11748545391599566No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5240.11703874116346337No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5230.11681538478719722No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5180.11569860290586646No Hit
GTCCAATTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGCAAGAT5160.11525189015333416No Hit
ACCCCAGAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTG5130.1145818210245357No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG5120.11435846464826956No Hit
ATCTTATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACC5120.11435846464826956No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5090.11368839551947109No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG5040.11257161363814033No Hit
ATCATCAACCTTCTCGAAGACTGGGGACCCTGTACCGAACATGGAGAACA5020.11212490088560803No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCC5020.11212490088560803No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5000.11167818813307573No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4960.11078476262801112No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGATGATATGGCTGAGACAGTA4950.11056140625174497No Hit
CTGTTAGGGGTCCCACAAATTGCTCACACCTATCAACAATGTCTTCCTGC4930.11011469349921266No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4860.1085511988653496No Hit
ATGCAGAGGAATATGATAAAACGCCGCAGATACATCCAGCGATAGCCAGG4850.10832784248908346No Hit
ATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCC4810.10743441698401884No Hit
ACCAAATACTTGCCCTTGGATAAAGGAATCAAACCCTATTATCCTGAGCA4790.10698770423148654No Hit
CATTATTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAA4790.10698770423148654No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACA4760.10631763510268809No Hit
ATTGTACACCTCACCATACGGCAATCAGGCAAGCTATTCTGTGTTGGGGT4760.10631763510268809No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG4670.10430742771629273No Hit
ATCCTGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAT4660.10408407134002659No Hit
GGCTGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC4640.10363735858749427No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4620.10319064583496197No Hit
GGAATATGGTGACCCGCAAAATGAGGCGCTACGTGTTGTCTCTCTTTTAT4610.10296728945869582No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4550.10162715120109891No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4540.10140379482483278No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGTTCCGGTGGGCGTTC4520.10095708207230045No Hit
GGCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGT4500.10051036931976816No Hit
CCTTTATCCAAGGGCAAGTATTTGGTAACTTTGGGATAAAATCTAGCAGG4500.10051036931976816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)