FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447715
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG17670.3946707168622896No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17460.38998023296070045No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT15530.3468724523413332No Hit
GTTGTGAGTATGCCCTGCGCCTGAGGGCTCCACCCCAACAGGCCCCCATG14540.32476017109098426No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14400.32163318182325806No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13060.29170342740359384No Hit
GAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCT11250.2512759232994204No Hit
CTCACCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTT10820.24167159911997588No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10810.24144824274370974No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA10610.2369811152183867No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAATCCACACAAGGA10060.22469651452374836No Hit
GGATAATAGGGTTTGATTCCTTTATCCAAGGGCAAGTATTTGGTAACTTT9970.222686307137353No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9960.22246295076108683No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9270.20705136079872238No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8920.19923388762940708No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT8700.19432004735155178No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACACACT8610.1923098399651564No Hit
CTATTATCCTGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT8460.18895949432116413No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGACAGCACGACAGAAT8420.18806606881609952No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8230.18382229766704264No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTACCGATTACATAACCCATGAA8000.17868510101292115No Hit
GTATTTGGTAACTTTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC7810.1744413298638643No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7680.1715376969724043No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCAATCAGGCA7480.1670705694470813No Hit
ATGTAAGACCTTGGGCAAGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC7470.16684721307081513No Hit
GGCTCTGAATGCAGGGTCCAATTGATGATCGGGGAAGAATCCCAGTGGAT7430.16595378756575052No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7420.16573043118948438No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7120.15902973990149985No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA7110.15880638352523369No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7060.15768960164390294No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6970.15567939425750757No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCGCGCATGCGCTGATGGCC6900.1541158996236445No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT6840.1527757613660476No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6820.15232904861351532No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC6790.15165897948471685No Hit
GTTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG6780.15143562310845068No Hit
TTGTACACCTCACCATACGGCAATCAGGCAAGCTATTCTGTGTTGGGGTG6770.15121226673218455No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6770.15121226673218455No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA6760.15098891035591838No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6560.14652178283059536No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6530.14585171370179692No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6470.14451157544419999No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6460.14428821906793385No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6400.14294808081033694No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6320.14116122980020773No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC6290.14049116067140927No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6260.13982109154261083No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6220.1389276660375462No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAAC6130.13691745865115085No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6120.13669410227488468No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6030.13468389488848934No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6020.1344605385122232No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6010.134237182135957No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5990.13379046938342473No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5950.1328970438783601No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG5940.13267368750209396No Hit
GTCCAAGGAATACTAACATTTAGATTCCCGAGAGTGAGATCTTCTGCGAC5920.13222697474956166No Hit
GCTCAGAATACTGTCTCAGCCATATCATCAACCTTCTCGAAGACTGGGGA5880.13133354924449706No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5870.13111019286823092No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5720.12775984722423864No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5710.12753649084797247No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5700.12731313447170634No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC5660.12641970896664173No Hit
GTAAACACACAGTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGC5620.12552628346157713No Hit
GTCCAATTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGCAAGAT5620.12552628346157713No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG5570.12440950158024637No Hit
CCTTATGAGTCCAAGGAATACTAACATTTAGATTCCCGAGAGTGAGATCT5560.12418614520398022No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5530.12351607607518175No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCAGG5500.12284600694638328No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5470.12217593781758485No Hit
CTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTT5460.12195258144131871No Hit
ATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGTTTT5390.12038908680745565No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5320.11882559217359256No Hit
CCCCAGAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTGT5320.11882559217359256No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5310.11860223579732643No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5280.11793216666852797No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT5250.11726209753972952No Hit
CCATATTCCTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5240.11703874116346337No Hit
ATCATCAACCTTCTCGAAGACTGGGGACCCTGTACCGAACATGGAGAACA5220.11659202841093107No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACA5210.1163686720346649No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5200.11614531565839875No Hit
GGTCTATGCCAAGTGTTTGCGGACGCAACCCCCACTGGTTGGGGCTTGGC5140.11480517740080184No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5120.11435846464826956No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG5120.11435846464826956No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG5050.1127949700144065No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG5050.1127949700144065No Hit
CCCCAACTACCGATTACATAACCCATGAAATTAAGGGAATATCCCCATCT5040.11257161363814033No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4960.11078476262801112No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4960.11078476262801112No Hit
GTTGACGATATGGCTGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGA4950.11056140625174497No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4940.11033804987547882No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4930.11011469349921266No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4800.10721106060775269No Hit
GTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACT4790.10698770423148654No Hit
ATCCTGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAT4780.1067643478552204No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT4750.10609427872642194No Hit
ACATAATTTGATTTTGTGTACAATATGTTCCTGTGGCAAGGAACCCCAAC4740.10587092235015579No Hit
ATTGTACACCTCACCATACGGCAATCAGGCAAGCTATTCTGTGTTGGGGT4740.10587092235015579No Hit
ACCCCAGAAGACCCACAATTCGTTGACACACTTTCCAATCAATAGGCCTG4710.10520085322135735No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGATGATATGGCTGAGACAGTA4710.10520085322135735No Hit
ATCTTATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACC4690.10475414046882503No Hit
CATTATTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAA4650.10386071496376044No Hit
CCCCAACAGGCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4590.10252057670616353No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4590.10252057670616353No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCC4550.10162715120109891No Hit
ATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGCC4510.10073372569603431No Hit
CTGTTAGGGGTCCCACAAATTGCTCACACCTATCAACAATGTCTTCCTGC4490.10028701294350201No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG4490.10028701294350201No Hit
CCCCATGGGGGCCTGTTGGGGTGGAGCCCTCAGGCGCAGGGCATACTCAC4480.10006365656723586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)