FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388210
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA17370.44743824218850625No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17010.43816491074418484No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15300.3941165863836583No Hit
GTCAAGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG14720.37917621905669613No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13260.3415677081991706No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12970.33409752453568947No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12120.31220215862548617No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG11110.2861853120733624No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10820.2787151284098813No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9110.23466680404935475No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA9070.23363643388887456No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8880.22874217562659388No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC8670.22333273228407305No Hit
GTGGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGG8610.22178717704335282No Hit
ATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGATTGACGA8560.2204992143427526No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC8360.2153473635403519No Hit
TGGAAAACATCGCATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCG8070.20787717987687077No Hit
AATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT8050.2073619947966307No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT8010.20633162463615054No Hit
CCTTTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT7680.19783107081218929No Hit
TCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGAC7460.19216403492954842No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT7370.1898457020684681No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG7180.18495144380618736No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7130.18366348110558717No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6960.17928440792354655No Hit
GCCTAGCAGCCATGGAAAGGACGTATACTTGCGGGAGAGCACGACAGAAT6830.17593570490198604No Hit
CCTCATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA6730.17335977950078565No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6570.16923829885886504No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6560.168980706318745No Hit
ACCCACACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAGTGGGAGCA6510.16769274361814482No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6350.1635712629762242No Hit
ATGCAAAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG6290.16202570773550398No Hit
TAGACAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC6240.1607377450349038No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6200.15970737487442363No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6180.15919218979418356No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6100.15713144947322324No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6070.15635867185286315No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6050.15584348677262308No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5990.15429793153190283No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5960.15352515391154273No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5750.14811571056902192No Hit
GTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG5610.14450941500734138No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTCAACAGTATC5380.1385847865845805No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5360.13806960150434042No Hit
ATGGTAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCC5340.13755441642410035No Hit
GTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5330.13729682388398032No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5330.13729682388398032No Hit
CTCAATTTTCTAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTC5300.13652404626362022No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATAACCCATGAA5280.13600886118338013No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5070.13059941784085932No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5050.13008423276061926No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5020.12931145514025913No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4960.1277658998995389No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4940.12725071481929884No Hit
CTCCCCCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAAGGCCCTGT4910.1264779371989387No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA4890.12596275211869865No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4850.1249323819582185No Hit
CTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACA4840.12467478941809845No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4840.12467478941809845No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4840.12467478941809845No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG4840.12467478941809845No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4780.12312923417737821No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4770.1228716416372582No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4770.1228716416372582No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4760.12261404909713815No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4750.12235645655701811No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4750.12235645655701811No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4650.11978053115581772No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4620.11900775353545762No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA4550.11720460575461734No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4490.1156590505138971No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACTT4460.114886272893537No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGT4450.11462868035341696No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4410.11359831019293681No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGA4400.11334071765281677No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG4390.11308312511269675No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTACACATCACCATACGG4350.11205275495221659No Hit
GAAAACTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTGTGAGG4340.11179516241209654No Hit
GGTAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC4320.11127997733185646No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4310.11102238479173644No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4310.11102238479173644No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4300.1107647922516164No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGTACAATATGTT4300.1107647922516164No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATAACCC4290.11050719971149635No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4280.1102496071713763No Hit
ATGCAAAGGAAGATGATAAAACGCCGCAGACACATCCAGTGATAACCAGG4280.1102496071713763No Hit
GGAACATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4240.10921923701089616No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4230.10896164447077614No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC4190.10793127431029598No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4190.10793127431029598No Hit
GAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4160.10715849668993585No Hit
ACATTCCCCCATGGGGAGGGATGAACCCTGGCCCAAATGCTCCCACTCCC4150.10690090414981583No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4090.10535534890909559No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4050.10432497874861545No Hit
GAGTTTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTTCCTTCCTGACTGC4050.10432497874861545No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA4030.10380979366837535No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4020.10355220112825533No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4000.10303701604801524No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4000.10303701604801524No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGTAGAGACAGTA3990.1027794235078952No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC3970.10226423842765514No Hit
GTGATGTGTACAATGTTCTGGAGACTCTAAGGCCTCCCGATACAGAGCAG3950.10174905334741505No Hit
GAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGACGCGGCGA3950.10174905334741505No Hit
GGGTAAAGGTTCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA3910.1007186831869349No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC3890.10020349810669484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)