FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388210
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA16740.43120991216094384No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14330.3691301099920147No Hit
GTCAAGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG14090.3629478890291337No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13730.35367455758481237No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT13720.35341696504469233No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12220.3147780840266866No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11450.29494345843744363No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11040.28438216429252205No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG10450.2691842044254399No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG10440.2689266118853198No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9480.24419772803379614No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA9180.236469951830195No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8950.23054532340743414No Hit
ATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGATTGACGA8080.2081347724169908No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8070.20787717987687077No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7860.20246773653434996No Hit
TGGAAAACATCGCATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCG7660.1973158857319492No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC7550.19448236779062877No Hit
AATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT7510.19345199763014861No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT7450.19190644238942842No Hit
CCTTTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACT7420.1911336647690683No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT7350.18933051698822803No Hit
TCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGAC7340.18907292444810797No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7070.18211792586486697No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6990.18005718554390665No Hit
GTGGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTCCCTTAGAGGTGG6740.1736173720409057No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6550.16872311377862495No Hit
GCCTAGCAGCCATGGAAAGGACGTATACTTGCGGGAGAGCACGACAGAAT6520.16795033615826485No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6010.15481311661214292No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6000.1545555240720229No Hit
TAGACAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC5960.15352515391154273No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5870.15120682105046238No Hit
ACCCACACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAGTGGGAGCA5800.14940367326962212No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5620.1447670075474614No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5590.1439942299271013No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5540.14270626722650112No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5520.14219108214626103No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5510.14193348960614102No Hit
GTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5450.14038793436542077No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5430.1398727492851807No Hit
ATGCAAAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG5430.1398727492851807No Hit
GTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG5420.13961515674506067No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATAACCCATGAA5370.13832719404446048No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5360.13806960150434042No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5210.13420571340253987No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5170.1331753432420597No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5160.13291775070193967No Hit
CTCAATTTTCTAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTC5140.1324025656216996No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5100.13137219546121945No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5030.12956904768037916No Hit
GTATCGGGAGGCCTTAGAGTCTCCAGAACATTGTACACATCACCATACGG5020.12931145514025913No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4990.12853867751989903No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4970.12802349243965896No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4900.12622034465881868No Hit
ATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGG4850.1249323819582185No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4840.12467478941809845No Hit
ATGGTAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCC4810.12390201179773835No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4810.12390201179773835No Hit
CGGTGGTTGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4760.12261404909713815No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4730.12184127147677802No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4690.12081090131629788No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATGGGTAACAGCGGCATAA4680.12055330877617784No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4670.12029571623605781No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4590.1182349759150975No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4530.11668942067437726No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA4430.1141134952731769No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATAACCC4410.11359831019293681No Hit
CTCCCCCACTTTCCACCAAACTCTTCAAGATCCCAGAGTCAAGGCCCTGT4400.11334071765281677No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4370.11256794003245665No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4300.1107647922516164No Hit
GAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4290.11050719971149635No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4260.10973442209113625No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGA4260.10973442209113625No Hit
GTGATGTGTACAATGTTCTGGAGACTCTAAGGCCTCCCGATACAGAGCAG4250.1094768295510162No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4240.10921923701089616No Hit
CTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACA4220.10870405193065609No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4160.10715849668993585No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4150.10690090414981583No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGTACAATATGTT4120.1061281265294557No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4110.10587053398933569No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGTAGAGACAGTA4100.10561294144921564No Hit
GAAAACTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTGTGAGG4090.10535534890909559No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4090.10535534890909559No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4050.10432497874861545No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTCAACAGTATC4050.10432497874861545No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC4040.1040673862084954No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4000.10303701604801524No Hit
GGAACATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4000.10303701604801524No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG3980.10252183096777517No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACTT3960.1020066458875351No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3940.10149146080729503No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA3930.10123386826717498No Hit
GGGTAAAGGTTCAGATACTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA3930.10123386826717498No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3920.10097627572705495No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3900.10046109064681488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)