FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573044
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA26380.4603485945232827No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC25150.4388842741569583No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG25060.4373137141301541No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC21500.37518933973656476No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20970.3659404862453843No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20690.3610542994953267No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA18860.32911957895030747No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT16730.29194965831594083No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA16620.2900300849498468No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15360.26804224457458764No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15200.265250137860269No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG15180.2649011245209792No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13950.2434368041546548No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC13440.2345369640027642No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13070.22808021722590235No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT12280.2142941903239542No Hit
AACCTAGACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT11320.19754155003804244No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG11210.1956219766719484No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC11160.19474944332372382No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG11020.19230634994869503No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10990.19178282993976029No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10600.18497706982360865No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10530.18375552313609425No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10380.18113792309142057No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT9870.17223808293952994No Hit
CTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGA9490.16560682949302322No Hit
GCCTAATCATCTCTTGTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGC9440.16473429614479865No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCCTCGAGGAGATTGATGATATGG9060.15810304269829192No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC8920.15565994932326313No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT8890.1551364293143284No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATTTGAACCTTTACCCCGTTGCC8850.15443840263574873No Hit
ATGTAAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8720.15216981593036485No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8500.14833066919817675No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8430.14710912251066235No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC8330.14536405581421322No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8300.14484053580527848No Hit
GCCACTCAGGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAG8270.14431701579634373No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAG8230.14361898911776408No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8020.1399543490552209No Hit
TATTTACATTGACATAGCTGACTACTGCTTCCCTGGATGCTGGGTCTTCC7860.13716224234090227No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7830.13663872233196753No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA7630.13314858893906925No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTGAC7590.1324505622604896No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7580.13227605559084468No Hit
CTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGGAGGACATT7560.13192704225155485No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC7550.13175253558190994No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAAAGCCTTTATATGCATGTATT7540.13157802891226503No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7520.1312290155729752No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7520.1312290155729752No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC7510.13105450890333029No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG7330.12791338884972184No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7320.12773888218007692No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG7250.12651733549256253No Hit
AAACCAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAG7230.1261683221532727No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7200.12564480214433796No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTG7180.12529578880504813No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7150.12477226879611339No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7120.12424874878717863No Hit
TATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAA7050.12302720209966425No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7000.12215466875143968No Hit
GGTCTTGCATGGTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6990.12198016208179475No Hit
CTGTTTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTG6920.12075861539428037No Hit
CCTGTTTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCT6890.12023509538534563No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6890.12023509538534563No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6780.11831552201925156No Hit
CATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG6750.11779200201031684No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGG6660.1162214419835126No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6660.1162214419835126No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG6640.11587242864422279No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC6610.11534890863528803No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6610.11534890863528803No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6600.11517440196564313No Hit
AGTTAAGGGAGTAGCCCCACCGTTTGGTTTTATTGGGGTTCAAATGTATA6580.11482538862635329No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6530.11395285527812872No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6500.11342933526919399No Hit
GTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAACTTGG6500.11342933526919399No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6470.11290581526025925No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6380.11133525523345503No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAGTG6330.11046272188523044No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG6330.11046272188523044No Hit
CTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6310.11011370854594063No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6300.10993920187629572No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6290.1097646952066508No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6280.10959018853700588No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6230.10871765518878132No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6200.10819413517984657No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6140.1071470951619771No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTA6120.10679808182268725No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGC6090.10627456181375253No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6070.10592554847446269No Hit
CTACTGTACCTGTCTTTAACCCTGATTGGCAAACTCCCTCCTTTCCTCAC6070.10592554847446269No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6060.10575104180481779No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTT6060.10575104180481779No Hit
CTTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAGATTCCTATGGGAG6030.10522752179588303No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTA6020.10505301512623813No Hit
GAATACATGCATATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAA6010.10487850845659322No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5990.10452949511730338No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAAATATTGTTTACAC5990.10452949511730338No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5990.10452949511730338No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA5980.10435498844765846No Hit
CATTTGAACCCCAATAAAACCAAACGGTGGGGCTACTCCCTTAACTTCAT5970.10418048177801356No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5970.10418048177801356No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC5960.10400597510836865No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5950.10383146843872372No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATTTGTGGGCCCTCT5920.10330794842978899No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5860.1022609084119195No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGGTGAGGCATAGCAGCAGGAT5840.10191189507262968No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5790.10103936172440511No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5760.10051584171547037No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCACAACATT5750.10034133504582546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)