FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573044
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG24310.42422571390678554No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA22080.38531072657596976No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20570.3589602194595877No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20400.3559936060756242No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC19330.33732139242361847No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18670.32580395222705416No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA17620.30748075191433816No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15850.2765930713871884No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT15630.2727539246550003No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA15040.2624580311459504No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14140.24675243087790819No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG13490.23540949735098873No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13170.22982528392235152No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12920.22546261718122868No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG11580.20207872344881023No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11430.19946112340413652No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11370.19841408338626704No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10650.18584960317183322No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG10480.18288298978786968No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC9900.17276160294846468No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9680.16892245621627658No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATCAATAATGTCCTCCTGT9660.16857344287698675No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9150.15967360272509615No Hit
GCCTAATCATCTCTTGTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGC8960.15635797600184279No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCCTCGAGGAGATTGATGATATGG8880.15496192264468348No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8840.15426389596610382No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAG8770.15304234927858942No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8650.15094826924285046No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8560.14937770921604623No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC8550.14920320254640132No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT8530.1488541892071115No Hit
CTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGA8130.14187392242131494No Hit
ATGTAAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8060.14065237573380054No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATTTGAACCTTTACCCCGTTGCC7900.13786026901948192No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7900.13786026901948192No Hit
AACCTAGACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT7900.13786026901948192No Hit
GCCACTCAGGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAG7790.13594069565338787No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7750.13524266897480822No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA7650.13349760227835908No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7560.13192704225155485No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAAAGCCTTTATATGCATGTATT7400.12913493553723623No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7370.1286114155283015No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7330.12791338884972184No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG7290.12721536217114218No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7210.12581930881398287No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTGAC7090.1237252287782439No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG7070.12337621543895409No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6900.12040960205499054No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6890.12023509538534563No Hit
TATTTACATTGACATAGCTGACTACTGCTTCCCTGGATGCTGGGTCTTCC6840.11936256203712106No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6840.11936256203712106No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6810.11883904202818632No Hit
TATAAAGGCTTTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAA6800.1186645353585414No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6680.11657045532280244No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6640.11587242864422279No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6610.11534890863528803No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6610.11534890863528803No Hit
GTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAGTAACTTGG6610.11534890863528803No Hit
CTGATTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGGAGGACATT6600.11517440196564313No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG6580.11482538862635329No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC6470.11290581526025925No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6460.11273130859061434No Hit
CTGTTTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTG6440.11238229525132451No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6380.11133525523345503No Hit
AAACCAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAG6350.11081173522452029No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6260.10924117519771606No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTA6250.10906666852807115No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6230.10871765518878132No Hit
CTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6200.10819413517984657No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGC6150.107321601831622No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAGTG6130.10697258849233218No Hit
CATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAG6130.10697258849233218No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTG6100.10644906848339744No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA6090.10627456181375253No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6080.1061000551441076No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6040.10540202846552796No Hit
CCTGTTTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCT6000.1047040017869483No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5990.10452949511730338No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5940.1036569617690788No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5930.1034824550994339No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5900.10295893509049915No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTA5890.10278442842085425No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5850.1020864017422746No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5850.1020864017422746No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5820.10156288173333984No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5800.10121386839405003No Hit
GGTCTTGCATGGTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5750.10034133504582546No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG5740.10016682837618053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)