FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390393
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT19220.4923244013084251No Hit
GTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG16320.4180402824845732No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA15910.4075380449956838No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15080.3862774178840297No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13890.35579531395286285No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11840.30328412650841585No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC11570.2963680188937814No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC10730.2748512396482519No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG10720.2745950875143765No Hit
CCCCACAAGACTGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG10650.27280202257724906No Hit
GCATTCCACCGCGTGGCCTGAGGATGACTTTCTCTTAGAGGTGGAGAGAT10520.26947204483686954No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGATAAGATTGACGATATGG10260.2628120893561104No Hit
ACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTG9920.25410291680434843No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT9510.24360067931545903No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG9410.2410391579767055No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9260.23719687596857525No Hit
TACAAGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACA9150.2343792024959464No Hit
GTTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA8940.229000007684564No Hit
CATACAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA8680.2223400522038049No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT8570.219522378731176No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT8500.21772931379404858No Hit
TTGCACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG8450.2164485531246718No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8440.21619240099079645No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGCTGCTGGCACT8420.21568009672304575No Hit
CATATAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGCCCCGTAC8380.21465548818754435No Hit
GGTTCTAGGAATCCTGATGTTGTGTTCTCCGTGTTCGGTGCAGGGTCCCC8360.21414318391979364No Hit
CATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTC8180.2095324455100373No Hit
CCGTTACATTGCACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTG7970.20415325069865495No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7730.1980055994856465No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7690.1969809909501451No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7430.19032103546938597No Hit
CCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTGTATTCC7390.18929642693388457No Hit
CCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTA7360.1885279705322585No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA7270.18622260132738036No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7200.1844295363902529No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7180.1839172321225022No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG7180.1839172321225022No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7130.18263647145312545No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA7120.1823803193192501No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG6950.1780257330433691No Hit
TTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6930.1775134287756184No Hit
CCCAAGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTT6830.1749519074368649No Hit
GGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG6670.17085347329485928No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6650.17034116902710858No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGCGGA6590.16880425622385647No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6580.16854810408998114No Hit
GTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6570.16829195195610577No Hit
CATATCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCC6490.16624273488510297No Hit
ACAATAGGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGC6310.16163199647534662No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG6220.15932662727046848No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC6120.15676510593171497No Hit
CTTTTGGAGTGTGGATTCGCACTCCTGCCGCTTACAGACCACAAAATGCC6010.1539474324590861No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5750.14728747697832698No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5740.14703132484445164No Hit
TCCACAGAGTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5610.14370134710407206No Hit
CCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5550.14216443430081993No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5510.14113982576531853No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGAAAGTCATCCT5490.14062752149756783No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT5460.1398590650959418No Hit
CTTGTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC5410.13857830442656502No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5390.13806600015881432No Hit
GTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTG5350.1370413916233129No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA5300.13576063095393617No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5260.13473602241843477No Hit
ATTTGCATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5170.1324306532135566No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5040.12910067547317702No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4960.12705145840217422No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4930.12628300200054815No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4890.12525839346504675No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4880.1250022413311714No Hit
AGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCGCTCCTAC4850.12423378492954534No Hit
GTATTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT4840.12397763279567002No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4800.1229530242601686No Hit
GCCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4770.12218456785854255No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4730.12115995932304115No Hit
GAGCAAAACTTATCGGAACCGACAACTCTGTTGTCCTCTCTCGGAAATAC4690.12013535078753974No Hit
GGTTTTAGGAATCCTGATGTTGTGTTCTCCGTGTTCGGTGCAGGGTCCCC4670.11962304651978904No Hit
AAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGA4670.11962304651978904No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4540.11629306877940947No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4500.11526846024390805No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATGTTGACAACAGTGCCAGC4500.11526846024390805No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4460.11424385170840665No Hit
ATCTTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4460.11424385170840665No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4450.1139876995745313No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT4440.11373154744065594No Hit
AACACGGAGAACACAACATCAGGATTCCTAGAACCCCTGCACGTGTTACA4400.11270693890515454No Hit
GGCCAATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG4390.11245078677127919No Hit
CATAAAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGCCCCGTAC4360.11168233036965315No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAAGTAATTTGG4290.1098892654325257No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC4240.10860850476314893No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC4220.10809620049539823No Hit
GGTGAACAATGTACCGGAGACTCTAAGGCCTCCCGATACAAAGCAGAGGC4210.10784004836152287No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG4190.10732774409377217No Hit
CCTGCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4180.10707159195989682No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4170.10681543982602147No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4160.10655928769214612No Hit
GTTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4140.10604698342439542No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4130.10579083129052007No Hit
GCATATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGG4110.10527852702276937No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAAGTAA4080.1045100706211433No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAATACAATA4060.1039977663533926No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT4060.1039977663533926No Hit
GGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCCTTG4020.1029731578178912No Hit
GTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCCGCCTGTAACACG3970.10169239714851444No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3970.10169239714851444No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3970.10169239714851444No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC3960.10143624501463909No Hit
GAACACGGAGAACACAACATCAGGATTCCTAGAACCCCTGCACGTGTTAC3950.10118009288076374No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3940.10092394074688839No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCA3910.10015548434526235No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3910.10015548434526235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)