FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390393
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT16780.42982328064283937No Hit
GTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG14890.381410527340398No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13350.34196309872359393No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA12880.3299239484314524No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12680.3248009057539454No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11260.28842730274364553No Hit
CCTGCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10850.27792506525475613No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC10540.2699843491046202No Hit
CCCCACAAGACTGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG10000.25615213387535124No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG9680.24795526559134004No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGATAAGATTGACGATATGG9560.2448814399848358No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC9460.2423199186460823No Hit
GCATTCCACCGCGTGGCCTGAGGATGACTTTCTCTTAGAGGTGGAGAGAT9280.23770918023632595No Hit
ACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTG9250.23694072383469988No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT9120.23361074609432034No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8220.21055705404553873No Hit
CATACAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA8130.2082516848406606No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG8120.2079955327067852No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8000.204921707100281No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7830.20056712082440004No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT7830.20056712082440004No Hit
GTTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA7630.195444078146893No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7530.1928825568081395No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGCTGCTGGCACT7380.18904027480000923No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7320.1875033619967571No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7290.18673490559513106No Hit
TACAAGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACA7170.18366107998862682No Hit
TTGCACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG7160.1834049278547515No Hit
GGTTCTAGGAATCCTGATGTTGTGTTCTCCGTGTTCGGTGCAGGGTCCCC7090.18161186291762405No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7000.17930649371274587No Hit
CATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTC7000.17930649371274587No Hit
CCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTGTATTCC6900.17674497237399237No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6840.17520805957074026No Hit
CATATAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGCCCCGTAC6820.17469575530298956No Hit
CCGTTACATTGCACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTG6800.17418345103523886No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6770.1734149946336128No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG6590.16880425622385647No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA6470.16573043061735226No Hit
GTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6380.1634250614124741No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA6380.1634250614124741No Hit
CCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTA6370.16316890927859873No Hit
CTTTTGGAGTGTGGATTCGCACTCCTGCCGCTTACAGACCACAAAATGCC6300.1613758443414713No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6290.16111969220759592No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA6280.16086354007372058No Hit
TTGTTCACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6270.16060738793984522No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG6080.15574049739621357No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC5940.15215436752195863No Hit
CATATCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCC5910.1513859111203326No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG5750.14728747697832698No Hit
GGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG5690.14575056417507487No Hit
CCCAAGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTT5660.1449821077734488No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5630.14421365137182277No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5450.13960291296206642No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT5330.1365290873555622No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5270.1349921745523101No Hit
TCCACAGAGTATGCAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5220.13371141388293337No Hit
ACAATAGGGAAACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGC5180.13268680534743196No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGAAAGTCATCCT5130.1314060446780552No Hit
GTATTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5120.13114989254417983No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGCGGA5090.1303814361425538No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5020.12858837120542632No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4980.12756376266992492No Hit
GAGCAAAACTTATCGGAACCGACAACTCTGTTGTCCTCTCTCGGAAATAC4830.12372148066179467No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4730.12115995932304115No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4700.1203915029214151No Hit
CCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4670.11962304651978904No Hit
GTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATTGTG4580.11731767731491087No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4570.11706152518103552No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA4540.11629306877940947No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4450.1139876995745313No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4410.11296309103902989No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAATACAATA4390.11245078677127919No Hit
GGTTTTAGGAATCCTGATGTTGTGTTCTCCGTGTTCGGTGCAGGGTCCCC4320.11065772183415175No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG4320.11065772183415175No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4300.11014541756640105No Hit
GCCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4290.1098892654325257No Hit
ATTTGCATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4280.10963311329865034No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4280.10963311329865034No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATGTTGACAACAGTGCCAGC4270.109376961164775No Hit
CTTGTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC4210.10784004836152287No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT4190.10732774409377217No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4100.10502237488889402No Hit
GCCCAAGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT4060.1039977663533926No Hit
GCATATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGG4020.1029731578178912No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4020.1029731578178912No Hit
AGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCGCTCCTAC4010.10271700568401584No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC3990.10220470141626514No Hit
GGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCCTTG3980.10194854928238979No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3930.10066778861301304No Hit
GTGCAGGGTCCCCAGTCCTCGATAAGATTGACGATATGGGTGAGACAGTA3930.10066778861301304No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCC3920.1004116364791377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)